. "Proteome analysis of the polyvalent bacteriophage 812"@en . . "Bakteriof\u00E1g 812 je polyvalentn\u00ED stafylokokov\u00FD f\u00E1g, lyzuj\u00EDc\u00ED \u0161irok\u00E9 spektrum druh\u016F rodu Staphylococcus, p\u0159ev\u00E1\u017En\u011B pak kmeny druhu S. aureus. Pro sv\u00E9 lytick\u00E9 vlastnosti je vhodn\u00FDm kandid\u00E1tem pro f\u00E1govou terapii, p\u0159i n\u00ED\u017E jsou bakteriof\u00E1gy vyu\u017E\u00EDv\u00E1ny pro l\u00E9\u010Dbu stafylokokov\u00FDch infekc\u00ED, p\u0159ev\u00E1\u017En\u011B t\u011Bch, kter\u00E9 jsou vyvol\u00E1ny kmeny rezistentn\u00EDmi k antibiotik\u016Fm. V t\u00E9to pr\u00E1ci se zam\u011B\u0159ujeme na anal\u00FDzu f\u00E1gov\u00E9ho proteomu, a to p\u0159edev\u0161\u00EDm na strukturn\u00ED a lytick\u00E9 proteiny, kter\u00E9 maj\u00ED vztah k rozmez\u00ED hostitele. F\u00E1g 812 byl pomno\u017Een na kmeni S. aureus SA812, purifikov\u00E1n ultracentrifugac\u00ED v gradientu CsCl a dialyzov\u00E1n proti vod\u011B. DNA uvoln\u011Bn\u00E1 z f\u00E1gov\u00FDch \u010D\u00E1stic byla odstran\u011Bna DN\u00E1zou I. F\u00E1gov\u00E9 proteiny byly denaturov\u00E1ny kr\u00E1tkodob\u00FDm pova\u0159en\u00EDm s pufrem obsahuj\u00EDc\u00EDm SDS a DTT a analyzov\u00E1ny jednorozm\u011Brnou elektrofor\u00E9zou na polyakrylamidov\u00E9m gelu. Po obarven\u00ED gelu pomoc\u00ED Coomassie brilliant blue a st\u0159\u00EDbra jsme nalezli 6 v\u00FDrazn\u00FDch pruh\u016F, kter\u00E9 jsme vy\u0159\u00EDzli z gelu, podrobili \u0161t\u011Bpen\u00ED trypsinem na specifick\u00E9 peptidy a d\u00E1le analyzovali"@cs . "Proteome analysis of the polyvalent bacteriophage 812"@en . "RIV/00216224:14310/04:00009921" . "6"^^ . "6"^^ . "Anal\u00FDza proteomu polyvalentn\u00EDho bakteriof\u00E1ga 812" . . "Anal\u00FDza proteomu polyvalentn\u00EDho bakteriof\u00E1ga 812" . "P(GA203/03/0515), Z(MSM 143100008)" . "2"^^ . . "VIII. Pracovn\u00ED setk\u00E1n\u00ED biochemik\u016F a molekul\u00E1rn\u00EDch biolog\u016F. Sborn\u00EDk abstrakt\u016F" . . "Kone\u010Dn\u00E1, Hana" . . "80-210-3321-5" . "RIV/00216224:14310/04:00009921!RIV08-MSM-14310___" . "2004-02-03+01:00"^^ . "Masarykova univerzita v Brn\u011B" . . . . . "Anal\u00FDza proteomu polyvalentn\u00EDho bakteriof\u00E1ga 812"@cs . "Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage is represented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. Comparison of the phage genomes revealed several rearrangements of DNA sequences as well as single nucleotide substitutions in different parts of the genomes. Nucleotide insertions and deletions of various size (~1 to 3 kb) were most frequently found in non-coding terminal genomic regions in phages with different host range. We tried to find possible correlations between mutational changes on DNA and proteome composition of the phages. After SDS-PAGE separation of phage virions digests 15 protein bands w"@en . "Anal\u00FDza proteomu polyvalentn\u00EDho bakteriof\u00E1ga 812"@cs . . . "Pant\u016F\u010Dek, Roman" . . "554523" . . "Preisler, Jan" . . . . "[F76A2BB836EE]" . "Brno" . . "Brno" . . "bacteriophage proteomics; MALDI-TOF; therapeutic bacteriphage; Staphylococcus bacteriophage"@en . . . "Do\u0161ka\u0159, Ji\u0159\u00ED" . "45-46" . "Bakteriof\u00E1g 812 je polyvalentn\u00ED stafylokokov\u00FD f\u00E1g, lyzuj\u00EDc\u00ED \u0161irok\u00E9 spektrum druh\u016F rodu Staphylococcus, p\u0159ev\u00E1\u017En\u011B pak kmeny druhu S. aureus. Pro sv\u00E9 lytick\u00E9 vlastnosti je vhodn\u00FDm kandid\u00E1tem pro f\u00E1govou terapii, p\u0159i n\u00ED\u017E jsou bakteriof\u00E1gy vyu\u017E\u00EDv\u00E1ny pro l\u00E9\u010Dbu stafylokokov\u00FDch infekc\u00ED, p\u0159ev\u00E1\u017En\u011B t\u011Bch, kter\u00E9 jsou vyvol\u00E1ny kmeny rezistentn\u00EDmi k antibiotik\u016Fm. V t\u00E9to pr\u00E1ci se zam\u011B\u0159ujeme na anal\u00FDzu f\u00E1gov\u00E9ho proteomu, a to p\u0159edev\u0161\u00EDm na strukturn\u00ED a lytick\u00E9 proteiny, kter\u00E9 maj\u00ED vztah k rozmez\u00ED hostitele. F\u00E1g 812 byl pomno\u017Een na kmeni S. aureus SA812, purifikov\u00E1n ultracentrifugac\u00ED v gradientu CsCl a dialyzov\u00E1n proti vod\u011B. DNA uvoln\u011Bn\u00E1 z f\u00E1gov\u00FDch \u010D\u00E1stic byla odstran\u011Bna DN\u00E1zou I. F\u00E1gov\u00E9 proteiny byly denaturov\u00E1ny kr\u00E1tkodob\u00FDm pova\u0159en\u00EDm s pufrem obsahuj\u00EDc\u00EDm SDS a DTT a analyzov\u00E1ny jednorozm\u011Brnou elektrofor\u00E9zou na polyakrylamidov\u00E9m gelu. Po obarven\u00ED gelu pomoc\u00ED Coomassie brilliant blue a st\u0159\u00EDbra jsme nalezli 6 v\u00FDrazn\u00FDch pruh\u016F, kter\u00E9 jsme vy\u0159\u00EDzli z gelu, podrobili \u0161t\u011Bpen\u00ED trypsinem na specifick\u00E9 peptidy a d\u00E1le analyzovali" . "Zdr\u00E1hal, Zbyn\u011Bk" . . . "14310" . "Eyer, Lud\u011Bk" . .