. "Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium" . . "7" . "14310" . "0949-8257" . "Brabec, Viktor" . "P(GA301/00/0556), P(GA305/02/1552), P(IAA5004101), P(NL6058), Z(AV0Z5004920)" . . "Monsu Scolaro, Luigi" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Novakova, Olga" . "Marini Palomeque, Mar\u00EDa Victoria" . "RIV/00216224:14310/02:00028886" . . . . . . . "Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium"@en . "Journal of Biological Inorganic Chemistry" . . . . "Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium"@en . . . . "6"^^ . "Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium" . . . "Romeo, Raffaello" . "[530A023C762A]" . . "Ka\u0161p\u00E1rkov\u00E1, Jana" . . "3"^^ . . "7" . "10"^^ . . . "Modifications of natural DNA by the dinuclear platinum(II) organometallic complex [{Pt(Me)Cl(Me2SO)}2(-N-N)] [where N-N=H2N(CH2)6NH2] (ORGANObisPt) were studied by methods of molecular biophysics. These methods include DNA binding studies using atomic absorption spectrophotometry, HPLC analysis of enzymatically digested DNA, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, mapping of DNA adducts by transcription assay, DNA melting curves measured by absorption spectrophotometry and conformational analysis of platinated DNA by differential pulse polarography. The results indicate that the complex ORGANObisPt binds irreversibly to DNA. Its DNA binding mode is, however, different from that of the formally equivalent [{cis-PtCl(NH3)2}2(-N-N)] (1,1/c,c), which exhibits antitumor activity including that in the tumor cells resistant to cisplatin."@en . . . "RIV/00216224:14310/02:00028886!RIV10-GA0-14310___" . "639728" . "DNA; Organoplatinum(II); Adducts; Cross-link; Conformation"@en . "Modifications of natural DNA by the dinuclear platinum(II) organometallic complex [{Pt(Me)Cl(Me2SO)}2(-N-N)] [where N-N=H2N(CH2)6NH2] (ORGANObisPt) were studied by methods of molecular biophysics. These methods include DNA binding studies using atomic absorption spectrophotometry, HPLC analysis of enzymatically digested DNA, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, mapping of DNA adducts by transcription assay, DNA melting curves measured by absorption spectrophotometry and conformational analysis of platinated DNA by differential pulse polarography. The results indicate that the complex ORGANObisPt binds irreversibly to DNA. Its DNA binding mode is, however, different from that of the formally equivalent [{cis-PtCl(NH3)2}2(-N-N)] (1,1/c,c), which exhibits antitumor activity including that in the tumor cells resistant to cisplatin." .