"Novotn\u00FD, Milos V." . "Vaughn, Jeffrey L." . "Pheromone binding by polymorphic mouse major urinary proteins"@en . "11" . . . . . . "Pheromone binding by polymorphic mouse major urinary proteins"@en . "Pheromone binding by polymorphic mouse major urinary proteins" . . "10"^^ . . "Mouse major urinary proteins (MUPs) have been proposed to play a role in regulat ing the release and capture of pheromones. Here, we report affinity measurements of five recombinant urinary MUP isoforms (MUPs-I, II, VII, VIII, and IX) and on e recombinant nasal isoform (MUP-IV) for each of three pheromonal ligands, (+/- )-2-sec-butyl-4,5-dihydrothiazole (SBT), 6-hydroxy-6-methyl-3-heptanone (HMH), a nd (+/-)dehydro-exo-brevicomin (DHB). Dissociation constants for all MUP-pherom one pairs were determined by isothermal titration calorimetry, and data for SBT were corroborated by measurements of intrinsic protein fluorescence. We also rep ort the isolation of MUP-IV protein from mouse nasal extracts, in which MUP-IV m RNA has been observed previously. The affinity of each MUP isoform for SBT (Kd ~ 0.04 to 0.9 uM) is higher than that for DHB (Kd ~ 26 to 58 uM), which in turn is higher than that for HMH (Kd ~ 50 to 200 uM). Isoforms I, II, VIII, a nd IX show very similar affinities for each of the ligands."@cs . . . "14310" . "RIV/00216224:14310/02:00007921!RIV08-GA0-14310___" . "Sharrow, Scott D." . . "P(GA203/00/0511)" . . "Protein Science" . . "Binding affinity; isothermal titration calorimetry; major urinary proteins eins; pheromones; selectivity"@en . "1"^^ . "2247" . "658159" . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Stone, Martin J." . . . "Mouse major urinary proteins (MUPs) have been proposed to play a role in regulat ing the release and capture of pheromones. Here, we report affinity measurements of five recombinant urinary MUP isoforms (MUPs-I, II, VII, VIII, and IX) and on e recombinant nasal isoform (MUP-IV) for each of three pheromonal ligands, (+/- )-2-sec-butyl-4,5-dihydrothiazole (SBT), 6-hydroxy-6-methyl-3-heptanone (HMH), a nd (+/-)dehydro-exo-brevicomin (DHB). Dissociation constants for all MUP-pherom one pairs were determined by isothermal titration calorimetry, and data for SBT were corroborated by measurements of intrinsic protein fluorescence. We also rep ort the isolation of MUP-IV protein from mouse nasal extracts, in which MUP-IV m RNA has been observed previously. The affinity of each MUP isoform for SBT (Kd ~ 0.04 to 0.9 uM) is higher than that for DHB (Kd ~ 26 to 58 uM), which in turn is higher than that for HMH (Kd ~ 50 to 200 uM). Isoforms I, II, VIII, a nd IX show very similar affinities for each of the ligands."@en . "5"^^ . . "Mouse major urinary proteins (MUPs) have been proposed to play a role in regulat ing the release and capture of pheromones. Here, we report affinity measurements of five recombinant urinary MUP isoforms (MUPs-I, II, VII, VIII, and IX) and on e recombinant nasal isoform (MUP-IV) for each of three pheromonal ligands, (+/- )-2-sec-butyl-4,5-dihydrothiazole (SBT), 6-hydroxy-6-methyl-3-heptanone (HMH), a nd (+/-)dehydro-exo-brevicomin (DHB). Dissociation constants for all MUP-pherom one pairs were determined by isothermal titration calorimetry, and data for SBT were corroborated by measurements of intrinsic protein fluorescence. We also rep ort the isolation of MUP-IV protein from mouse nasal extracts, in which MUP-IV m RNA has been observed previously. The affinity of each MUP isoform for SBT (Kd ~ 0.04 to 0.9 uM) is higher than that for DHB (Kd ~ 26 to 58 uM), which in turn is higher than that for HMH (Kd ~ 50 to 200 uM). Isoforms I, II, VIII, a nd IX show very similar affinities for each of the ligands." . "11" . "\u017D\u00EDdek, Luk\u00E1\u0161" . "Pheromone binding by polymorphic mouse major urinary proteins"@cs . "0961-8368" . "[BF1E70414CFE]" . "Pheromone binding by polymorphic mouse major urinary proteins" . "Pheromone binding by polymorphic mouse major urinary proteins"@cs . "RIV/00216224:14310/02:00007921" .