"RIV/00216224:14310/02:00006229" . . . "The latest development in derivatization in capillary electrophoresis is on-column mode. In this set-up a derivatization reaction is performed during the electrophoretic separation. In principle, the front end of the capillary is used as the reaction chamber. The analyte and the reagent are injected separately and are mixed because of their different electrophoretic mobilities. Various on-column reactions have been demonstrated so far however only for derivatization of the amino group containing compounds. In all these applications the simplified derivatization scheme is the same. The detector nonresponding analytes A, B and C are converted into the derivatives AX, BX and CX that have enhanced detector response properties, in the chemical reaction with the derivatization reagent X : A + B + C + .... + X > AX + BX + CX + .... In this study a new type of on-column reaction detection is reported. The analytes A, B, and C react with the reagent XX forming the derivatives AX, BX and CX, and a reaction b"@en . "Kapil\u00E1rn\u00ED z\u00F3nov\u00E1 elektrofor\u00E9za je relativn\u011B nov\u00E1 analytick\u00E1 metoda, je\u017E v sou\u010Dasn\u00E9 dob\u011B zaznamen\u00E1v\u00E1 obrovsk\u00FD rozvoj. Jej\u00ED hlavn\u00ED v\u00FDhodou je zna\u010Dn\u00E1 diverzita v aplika\u010Dn\u00EDch mo\u017Enostech, umo\u017E\u0148uje anal\u00FDzu n\u00EDzkomolekul\u00E1rn\u00EDch i vysokomolekul\u00E1rn\u00EDch l\u00E1tek, anal\u00FDzu l\u00E1tek iontov\u00E9ho i neiontov\u00E9ho charakteru. Pou\u017Eit\u00E9 mechanismy, kter\u00E9 se u t\u00E9to metody uplat\u0148uj\u00ED jsou p\u0159itom odli\u0161n\u00E9 od mechanism\u016F uplat\u0148ovan\u00FDch p\u0159i chromatografick\u00FDch anal\u00FDz\u00E1ch, co\u017E z n\u00ED d\u011Bl\u00E1 komplement\u00E1rn\u00ED analytickou metodu vzhledem k chromatografii. Kapil\u00E1rn\u00ED z\u00F3nov\u00E1 elektrofor\u00E9za p\u0159inesla \u0159adu dal\u0161\u00EDch v\u00FDhod - vysokou \u00FA\u010Dinnost a rychlost anal\u00FDzy, mal\u00E9 mno\u017Estv\u00ED vzorku a pou\u017Eit\u00E9ho pufru a s t\u00EDm souvisej\u00EDc\u00ED mal\u00E9 mno\u017Estv\u00ED odpad\u016F. \u00DAzk\u00FD pr\u016Fm\u011Br separa\u010Dn\u00ED kapil\u00E1ry, kter\u00FD umo\u017E\u0148uje ji\u017E zmi\u0148ovan\u00E9 v\u00FDhody, je v\u0161ak zodpov\u011Bdn\u00FD za podstatnou limitaci t\u00E9to metody, kterou je n\u00EDzk\u00E1 koncentra\u010Dn\u00ED citlivost. P\u0159esto\u017Ee absolutn\u00ED citlivost je d\u00EDky mal\u00FDm d\u00E1vkovan\u00FDm objem\u016Fm extr\u00E9mn\u011B vysok\u00E1, koncentra\u010Dn\u00ED citlivost je p\u0159edev\u0161\u00EDm p\u0159i pou\u017Eit\u00ED spektrofotometrick\u00E9 detekce ni\u017E\u0161\u00ED ne\u017E"@cs . "Olivov\u00E1, Petra" . "Olomouc" . . "On-column derivatizace v kapil\u00E1rn\u00ED z\u00F3nov\u00E9 elektrofor\u00E9ze"@cs . "P(GA525/00/0785)" . "On-column derivatizace v kapil\u00E1rn\u00ED z\u00F3nov\u00E9 elektrofor\u00E9ze"@cs . . . "RIV/00216224:14310/02:00006229!RIV09-GA0-14310___" . "June 24-27., 2002, Olomouc" . "On-column derivatization in CZE"@en . "2"^^ . "[E4E463CE5914]" . "derivatization; on-column; CZE"@en . . . "On-column derivatizace v kapil\u00E1rn\u00ED z\u00F3nov\u00E9 elektrofor\u00E9ze" . . "Kapil\u00E1rn\u00ED z\u00F3nov\u00E1 elektrofor\u00E9za je relativn\u011B nov\u00E1 analytick\u00E1 metoda, je\u017E v sou\u010Dasn\u00E9 dob\u011B zaznamen\u00E1v\u00E1 obrovsk\u00FD rozvoj. Jej\u00ED hlavn\u00ED v\u00FDhodou je zna\u010Dn\u00E1 diverzita v aplika\u010Dn\u00EDch mo\u017Enostech, umo\u017E\u0148uje anal\u00FDzu n\u00EDzkomolekul\u00E1rn\u00EDch i vysokomolekul\u00E1rn\u00EDch l\u00E1tek, anal\u00FDzu l\u00E1tek iontov\u00E9ho i neiontov\u00E9ho charakteru. Pou\u017Eit\u00E9 mechanismy, kter\u00E9 se u t\u00E9to metody uplat\u0148uj\u00ED jsou p\u0159itom odli\u0161n\u00E9 od mechanism\u016F uplat\u0148ovan\u00FDch p\u0159i chromatografick\u00FDch anal\u00FDz\u00E1ch, co\u017E z n\u00ED d\u011Bl\u00E1 komplement\u00E1rn\u00ED analytickou metodu vzhledem k chromatografii. Kapil\u00E1rn\u00ED z\u00F3nov\u00E1 elektrofor\u00E9za p\u0159inesla \u0159adu dal\u0161\u00EDch v\u00FDhod - vysokou \u00FA\u010Dinnost a rychlost anal\u00FDzy, mal\u00E9 mno\u017Estv\u00ED vzorku a pou\u017Eit\u00E9ho pufru a s t\u00EDm souvisej\u00EDc\u00ED mal\u00E9 mno\u017Estv\u00ED odpad\u016F. \u00DAzk\u00FD pr\u016Fm\u011Br separa\u010Dn\u00ED kapil\u00E1ry, kter\u00FD umo\u017E\u0148uje ji\u017E zmi\u0148ovan\u00E9 v\u00FDhody, je v\u0161ak zodpov\u011Bdn\u00FD za podstatnou limitaci t\u00E9to metody, kterou je n\u00EDzk\u00E1 koncentra\u010Dn\u00ED citlivost. P\u0159esto\u017Ee absolutn\u00ED citlivost je d\u00EDky mal\u00FDm d\u00E1vkovan\u00FDm objem\u016Fm extr\u00E9mn\u011B vysok\u00E1, koncentra\u010Dn\u00ED citlivost je p\u0159edev\u0161\u00EDm p\u0159i pou\u017Eit\u00ED spektrofotometrick\u00E9 detekce ni\u017E\u0161\u00ED ne\u017E" . . . "2"^^ . "2002-01-01+01:00"^^ . . . "80-86238-24-5" . "On-column derivatizace v kapil\u00E1rn\u00ED z\u00F3nov\u00E9 elektrofor\u00E9ze" . "14310" . "1"^^ . . "\u010Cesk\u00E1 spole\u010Dnost chemick\u00E1 - skupina pro chromatografii a elektrofor\u00E9zu" . . . "657037" . "Book of abstracts of Developments in Chromatography and Electrophoresis & Chiranal 2002" . "On-column derivatization in CZE"@en . "Glatz, Zden\u011Bk" . . .