. "\u0160imek, Vladim\u00EDr" . . "\u0160tika, Ji\u0159\u00ED" . "\u010Cesk\u00E9 Bud\u011Bjovice" . "725580" . "\u010Cesk\u00E9 Bud\u011Bjovice" . . . . . . "Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60" . . "14310" . . . "2000-01-01+01:00"^^ . . . "Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60"@en . "80-7232-120-X" . . "Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60" . "Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60"@en . "Cells II" . "Tumor necrosis factor-a (TNF-a) has been found to reduce cellular proliferation and viability, and to induce apoptosis, as well as a partial differentiation. These effects could contribute to elimination of leukemic cells. Using human leukemic cell line HL-60 as a model, we administered cyclooxygenase (indomethacin) and lipoxygenase (MK-886) inhibitors together with TNF-a, in order to evaluate the possible role of prostaglandins and leukotrienes in these processes. The following methods were used to assess cell proliferation, differentiation and apoptosis: cells were counted with haemocytometer; apoptosis and cell cycle were quantified using flowcytometry and fluorescence microscopy; viability was detected by means of light microscopy and flowcytometry; differentiation was followed as nonspecific esterase activity. Our results indicate that indomethacin can potentiate TNF-a-induced apoptosis, resulting in the decrease of number of cells and their viability during simultaneous treatment with TNF-a."@en . "5"^^ . . . "Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60"@en . "0"^^ . "0"^^ . "Vondr\u00E1\u010Dek, Jan" . "Hofmanov\u00E1, Ji\u0159ina" . "RIV/00216224:14310/00:00003964" . . "Tumor necrosis factor-a (TNF-a) has been found to reduce cellular proliferation and viability, and to induce apoptosis, as well as a partial differentiation. These effects could contribute to elimination of leukemic cells. Using human leukemic cell line HL-60 as a model, we administered cyclooxygenase (indomethacin) and lipoxygenase (MK-886) inhibitors together with TNF-a, in order to evaluate the possible role of prostaglandins and leukotrienes in these processes. The following methods were used to assess cell proliferation, differentiation and apoptosis: cells were counted with haemocytometer; apoptosis and cell cycle were quantified using flowcytometry and fluorescence microscopy; viability was detected by means of light microscopy and flowcytometry; differentiation was followed as nonspecific esterase activity. Our results indicate that indomethacin can potentiate TNF-a-induced apoptosis, resulting in the decrease of number of cells and their viability during simultaneous treatment with TNF-a." . "Kopp nakladatelstv\u00ED" . "[9C71C82709CB]" . "RIV/00216224:14310/00:00003964!RIV/2002/GA0/143102/N" . "Kozub\u00EDk, Alois" . . "1"^^ . "2"^^ . "135" . "P(GA524/99/0694)" .