. "Pant\u016F\u010Dek, Roman" . "723190" . "9"^^ . . "Masarykova univerzita" . . "Mal\u00E1, Zdena" . . "2"^^ . "Bo\u010Dek, Petr" . . "P\u0159\u00EDprava specifick\u00FDch sond pro detekci prof\u00E1g\u016F seroskupin A, B a F v lyzogenn\u00EDch kmenech Staphylococcus aureus"@cs . "47" . "RIV/00216224:14310/00:00002001!RIV/2002/MSM/143102/N" . "10"^^ . "P\u0159\u00EDprava specifick\u00FDch sond pro detekci prof\u00E1g\u016F seroskupin A, B a F v lyzogenn\u00EDch kmenech Staphylococcus aureus" . . "80-210-2266-3" . . . "Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus"@en . "R\u016F\u017Ei\u010Dkov\u00E1, Vladislava" . "RIV/00216224:14310/00:00002001" . "Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus"@en . "P\u0159\u00EDprava specifick\u00FDch sond pro detekci prof\u00E1g\u016F seroskupin A, B a F v lyzogenn\u00EDch kmenech Staphylococcus aureus"@cs . "P\u0159\u00EDprava specifick\u00FDch sond pro detekci prof\u00E1g\u016F seroskupin A, B a F v lyzogenn\u00EDch kmenech Staphylococcus aureus" . "Na z\u00E1klad\u011B kvantitativn\u00ED HindIII-restrik\u010Dn\u00ED anal\u00FDzy genomov\u00E9 DNA byla stanovena genetick\u00E1 p\u0159\u00EDbuznost bakteriof\u00E1g\u016F S. aureus mezin\u00E1rodn\u00ED standardn\u00ED \u0159ady. F\u00E1gy byly rozd\u011Bleny do p\u011Bti klastr\u016F ozna\u010Den\u00FDch A, F, Ba, Bb a Bc. Klastry A a F obsahuj\u00ED v\u0161echny f\u00E1gy seroskupin A a F a odpov\u00EDdaj\u00ED f\u00E1gov\u00FDm druh\u016Fm 3A a 77 navr\u017Een\u00FDm Ackermanem a DuBowen (1987). F\u00E1gy seroskupiny B byly rozd\u011Bleny do t\u0159\u00ED samostatn\u00FDch klastr\u016F (Ba, Bb a Bc), kter\u00E9 se v\u00FDznamn\u011B li\u0161\u00ED sv\u00FDmi restrik\u010Dn\u00EDmi spektry, co\u017E sv\u011Bd\u010D\u00ED o jejich p\u0159\u00EDslu\u0161nosti k v\u00EDce f\u00E1gov\u00FDm druh\u016Fm. Pomoc\u00ED hybridiza\u010Dn\u00ED anal\u00FDzy byly identifikov\u00E1ny spole\u010Dn\u00E9 HindIII-restrik\u010Dn\u00ED fragmenty specifick\u00E9 pro ka\u017Edou z f\u00E1gov\u00FDch seroskupin A, B a F. Z restrik\u010Dn\u00EDch fragment\u016F f\u00E1ga 3A (1700 bp), f\u00E1ga 53 (4060 bp) a f\u00E1ga 77 (8300 bp) byly p\u0159ipraveny specifick\u00E9 sondy umo\u017E\u0148uj\u00EDc\u00ED detekci prof\u00E1g\u016F p\u0159\u00EDslu\u0161n\u00FDch seroskupin v lyzogenn\u00EDch kmenech S. aureus. Vysok\u00E1 \u00FA\u010Dinnost sond byla prov\u011B\u0159ena u experiment\u00E1ln\u011B p\u0159ipraven\u00FDch lyzogenn\u00EDch kmen\u016F, u nich\u017E se poda\u0159ilo identifikovat a\u017E t\u0159i r\u016Fzn\u00E9 prof\u00E1gy. Pomoc\u00ED" . "[2CF977AAB879]" . "P(GA301/97/1192), Z(AV0Z4031919), Z(MSM 143100008)" . . "4th Workshop of Biochemists and Molecular Biologists. Biochemistry and molecular biology on the threshold of a new century" . "Rosypal, Stanislav" . "Pallov\u00E1, Petra" . . "14310" . . . . . "Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus"@en . . . "Kailerov\u00E1, Jana" . . "On the basis of quantitative HindIII-restriction analysis of genomic DNAs, the S. aureus bacteriophages of International Typing Set were divided into five clusters designated as A, F, Ba, Bb and Bc. The clusters A and F include all the phages of serogroups A and F and correspond with the species 3A and 77 proposed by Ackermann and DuBow (1987). On the other hand, the phages of serogroup B were divided into three clusters designated as Ba, Bb and Bc that differ significantly each from the other in their restriction pattern. The clusters Ba and Bb may represent two separate species, while the cluster Bc, formed at a low similarity level, may include more than one phage species. For each of the phage serogroups A, B and F, the common HindIII-restriction fragments were identified of which those of phage 3A (1700 bp), of 53 (4060 bp) and of 77 (8300 bp) were used for the preparation of probes specific to the phages of serogroups A, B and F. These probes have been shown to be very effective, it making possi"@en . "2000-01-01+01:00"^^ . . "Do\u0161ka\u0159, Ji\u0159\u00ED" . "Pant\u016F\u010Dkov\u00E1, Pavla" . "0"^^ . . . "Klep\u00E1rn\u00EDk, Karel" . "0"^^ . "Brno" . "Brno" . . . . . . .