"H\u00E9\u017Eov\u00E1, Renata" . "5"^^ . . "Mediators of Inflammation" . "2014" . "10"^^ . . "000334283100001" . . "microRNA; dendritic cells; expression profiling"@en . . . "14110" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "P(ED1.1.00/02.0068), P(NT13514), P(NT13547)" . "29207" . . "[AF209607CEB8]" . "RIV/00216224:14110/14:00077328!RIV15-MSM-14110___" . . "259689" . . . "Mich\u00E1lek, Jaroslav" . . "RIV/00216224:14110/14:00077328" . "MicroRNA profiling of activated and tolerogenic human dendritic cells"@en . "MicroRNA profiling of activated and tolerogenic human dendritic cells"@en . "Dendritic cells (DCs) belong to the immune system and are particularly studied for their potential to direct either an activated or tolerogenic immune response. The roles of microRNAs (miRNAs) in posttranscriptional gene expression regulation are being increasingly investigated. This study's aim is to evaluate the miRNAs' expression changes in prepared human immature (iDCs), activated (aDCs), and tolerogenic dendritic cells (tDCs). The dendritic cells were prepared using GM-CSF and IL-4 (iDC) and subsequently maturated by adding LPS and IFN-gamma(aDC) or IL-10 and TGF-beta(tDC). Surface markers, cytokine profiles, and miRNA profiles were evaluated in iDC, tDC, and aDC at 6 h and 24 h of maturation. We identified 4 miRNAs (miR-7, miR-9, miR-155 and miR-182), which were consistently overexpressed in aDC after 6 h and 24 h of maturation and 3 miRNAs (miR-17, miR-133b, and miR-203) and miR-23b cluster solely expressed in tDC." . "MicroRNA profiling of activated and tolerogenic human dendritic cells" . . "Meli, Albano" . . . . . . "Dendritic cells (DCs) belong to the immune system and are particularly studied for their potential to direct either an activated or tolerogenic immune response. The roles of microRNAs (miRNAs) in posttranscriptional gene expression regulation are being increasingly investigated. This study's aim is to evaluate the miRNAs' expression changes in prepared human immature (iDCs), activated (aDCs), and tolerogenic dendritic cells (tDCs). The dendritic cells were prepared using GM-CSF and IL-4 (iDC) and subsequently maturated by adding LPS and IFN-gamma(aDC) or IL-10 and TGF-beta(tDC). Surface markers, cytokine profiles, and miRNA profiles were evaluated in iDC, tDC, and aDC at 6 h and 24 h of maturation. We identified 4 miRNAs (miR-7, miR-9, miR-155 and miR-182), which were consistently overexpressed in aDC after 6 h and 24 h of maturation and 3 miRNAs (miR-17, miR-133b, and miR-203) and miR-23b cluster solely expressed in tDC."@en . "Slab\u00FD, Ond\u0159ej" . . "5"^^ . . . . "0962-9351" . "MicroRNA profiling of activated and tolerogenic human dendritic cells" . "10.1155/2014/259689" . "\u0160tumpfov\u00E1, Zuzana" .