"Krut\u00E1, Miriama" . "Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts" . "14110" . "mutation frequency; human embryonic stem cells; induced pluripotent stem cells; hypoxanthine phosphoribosyltransferase; base excision repair; apurinic/apyrimidinic endonuclease"@en . "The genomic destabilization associated with the adaptation of human embryonic stem cells (hESCs) to culture conditions or the reprogramming of induced pluripotent stem cells (iPSCs) increases the risk of tumorigenesis upon the clinical use of these cells and decreases their value as a model for cell biology studies. Base excision repair (BER), a major genomic integrity maintenance mechanism, has been shown to fail during hESC adaptation. Here, we show that the increase in the mutation frequency (MF) caused by the inhibition of BER was similar to that caused by the hESC adaptation process. The increase in MF reflected the failure of DNA maintenance mechanisms and the subsequent increase in MF rather than being due solely to the accumulation of mutants over a prolonged period, as was previously suggested. The increase in the ionizing radiation-induced MF in adapted hESCs exceeded the induced MF in non-adapted hESCs and differentiated cells." . . . . . "Rotrekl, Vladim\u00EDr" . "B\u00E1rtov\u00E1, Eva" . "12"^^ . . "Ko\u0161kov\u00E1, Stanislava" . "Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts"@en . . . "10"^^ . "14"^^ . . . . "P(EE2.3.30.0009), P(GA13-19910S), P(GBP302/12/G157), Z(MSM0021622430)" . . . "\u0160eneklov\u00E1, Monika" . "Salykin, Anton" . "Stem Cells and Development" . "20" . "Neelsen, Kai J." . . "23" . "31200" . . "Zerz\u00E1nkov\u00E1, Lenka" . . "000342614300004" . "Franek, Michal" . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . "Baumeisterov\u00E1, Aneta" . "The genomic destabilization associated with the adaptation of human embryonic stem cells (hESCs) to culture conditions or the reprogramming of induced pluripotent stem cells (iPSCs) increases the risk of tumorigenesis upon the clinical use of these cells and decreases their value as a model for cell biology studies. Base excision repair (BER), a major genomic integrity maintenance mechanism, has been shown to fail during hESC adaptation. Here, we show that the increase in the mutation frequency (MF) caused by the inhibition of BER was similar to that caused by the hESC adaptation process. The increase in MF reflected the failure of DNA maintenance mechanisms and the subsequent increase in MF rather than being due solely to the accumulation of mutants over a prolonged period, as was previously suggested. The increase in the ionizing radiation-induced MF in adapted hESCs exceeded the induced MF in non-adapted hESCs and differentiated cells."@en . "Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts" . . "[68DE0550C59F]" . "10.1089/scd.2013.0611" . "RIV/00216224:14110/14:00073651" . "Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts"@en . . "RIV/00216224:14110/14:00073651!RIV15-MSM-14110___" . . "Hampl, Ale\u0161" . "Dvo\u0159\u00E1k, Petr" . . "Ra\u0161ka, Jan" . . . "Pe\u0161l, Martin" . . . . "1547-3287" . . . .