"8"^^ . . . "Kryukov, Fedor" . . "0007-1048" . . . "9"^^ . "RIV/00216224:14110/14:00073562" . "\u0158\u00EDhov\u00E1, Lucie" . . "Gre\u0161likov\u00E1, Henrieta" . "nestin; multiple myeloma; monoclonal gammopathy; stem cells; flow cytometry"@en . "Nestin expression throughout multistep pathogenesis of multiple myeloma" . . . . "000331354700011" . . . . "I, P(GAP304/10/1395), P(NT11154), P(NT13190), Z(MSM0021622434)" . . . . . . "\u0160ev\u010D\u00EDkov\u00E1, Sabina" . "British journal of haematology" . . . "32185" . "Nestin expression throughout multistep pathogenesis of multiple myeloma"@en . "The stem cell marker nestin (NES) is found in dividing cells of developing and regenerating tissues. Upon terminal differentiation, NES expression is diminished but may be re-expressed following injury or in cancer. Surprisingly, we recently confirmed NES as a tumour-specific marker for mature CD138(+)38(+) plasma cells (PC) in multiple myeloma (MM). The present study analysed NES expression throughout the spectrum of MM developmental stages, starting with individuals with no haematological malignancy, through monoclonal gammopathy of undetermined significance (MGUS) and MM to plasma cell leukaemia (PCL) and MM cell lines. NES was analysed in bone marrow PC of 163 MM, four PCL and nine MGUS patients, 10 individuals with no haematological malignancy and 6 myeloma cell lines (OPM-2, RPMI-8226, MOLP-8, U-266, EJM, NCI-H929) by flow cytometry and/or real-time polymerase chain reaction or immunochemistry. We observed a tendency of increased NES expression in parallel with disease progression." . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "10.1111/bjh.12689" . "Nestin expression throughout multistep pathogenesis of multiple myeloma" . "[B14C7CDD60CC]" . "RIV/00216224:14110/14:00073562!RIV15-MSM-14110___" . "Kryukova, Elena Vladimirovna" . . "Nestin expression throughout multistep pathogenesis of multiple myeloma"@en . . "5" . "The stem cell marker nestin (NES) is found in dividing cells of developing and regenerating tissues. Upon terminal differentiation, NES expression is diminished but may be re-expressed following injury or in cancer. Surprisingly, we recently confirmed NES as a tumour-specific marker for mature CD138(+)38(+) plasma cells (PC) in multiple myeloma (MM). The present study analysed NES expression throughout the spectrum of MM developmental stages, starting with individuals with no haematological malignancy, through monoclonal gammopathy of undetermined significance (MGUS) and MM to plasma cell leukaemia (PCL) and MM cell lines. NES was analysed in bone marrow PC of 163 MM, four PCL and nine MGUS patients, 10 individuals with no haematological malignancy and 6 myeloma cell lines (OPM-2, RPMI-8226, MOLP-8, U-266, EJM, NCI-H929) by flow cytometry and/or real-time polymerase chain reaction or immunochemistry. We observed a tendency of increased NES expression in parallel with disease progression."@en . . . "Kubiczkov\u00E1, Lenka" . . . "14110" . "H\u00E1jek, Roman" . . "164" . "\u0160v\u00E1chov\u00E1, Hana" . "N\u011Bmec, Pavel" . . . "9"^^ .