"Nonlinear Regression Models for Determination of Nicotinamide Adenine Dinucleotide Content in Human Embryonic Stem Cells"@en . . "000327386500005" . "P(ED1.100/02/0123), P(GAP206/11/0009), Z(MSM0021622430)" . "Nonlinear Regression Models for Determination of Nicotinamide Adenine Dinucleotide Content in Human Embryonic Stem Cells" . "[BF6BC0BFE4B9]" . . . "Nonlinear Regression Models for Determination of Nicotinamide Adenine Dinucleotide Content in Human Embryonic Stem Cells"@en . . . . . "Kyrylenko, Sergiy" . "Salykin, Anton" . . . . "14110" . . "Kyrylenko, Olga" . . "Glatz, Zden\u011Bk" . "1550-8943" . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Nonlinear Regression Models for Determination of Nicotinamide Adenine Dinucleotide Content in Human Embryonic Stem Cells" . "RIV/00216224:14110/13:00066458" . "Dvo\u0159\u00E1k, Petr" . "91975" . . "Musilov\u00E1, Jindra" . "6" . "8"^^ . . "Recent evidence suggests that energy metabolism contributes to molecular mechanisms controlling stem cell identity. For example, human embryonic stem cells (hESCs) receive their metabolic energy mostly via glycolysis rather than mitochondrial oxidative phosphorylation. This suggests a connection of metabolic homeostasis to stemness. Nicotinamide adenine dinucleotide (NAD) is an important cellular redox carrier and a cofactor for various metabolic pathways, including glycolysis. Therefore, accurate determination of NAD cellular levels and dynamics is of growing importance for understanding the physiology of stem cells. Conventional analytic methods for the determination of metabolite levels rely on linear calibration curves. However, in actual practice many two-enzyme cycling assays, such as the assay systems used in this work, display prominently nonlinear behavior." . . "Recent evidence suggests that energy metabolism contributes to molecular mechanisms controlling stem cell identity. For example, human embryonic stem cells (hESCs) receive their metabolic energy mostly via glycolysis rather than mitochondrial oxidative phosphorylation. This suggests a connection of metabolic homeostasis to stemness. Nicotinamide adenine dinucleotide (NAD) is an important cellular redox carrier and a cofactor for various metabolic pathways, including glycolysis. Therefore, accurate determination of NAD cellular levels and dynamics is of growing importance for understanding the physiology of stem cells. Conventional analytic methods for the determination of metabolite levels rely on linear calibration curves. However, in actual practice many two-enzyme cycling assays, such as the assay systems used in this work, display prominently nonlinear behavior."@en . . . . "Kuzmic, Petr" . "9" . . . . . "5"^^ . . "RIV/00216224:14110/13:00066458!RIV14-MSM-14110___" . . "10.1007/s12015-013-9454-3" . "Stem Cell Reviews and Reports" . "Biochemical assay; Enzymatic cycling; hESC-derived fibroblasts; Human embryonic stem cells; NAD; Nonlinear regression"@en . "7"^^ . .