"11" . "14110" . "Clinical implication of centrosome amplification and expression of centrosomal functional genes in multiple myeloma" . . "9"^^ . . . "Clinical implication of centrosome amplification and expression of centrosomal functional genes in multiple myeloma" . . "8"^^ . . "Clinical implication of centrosome amplification and expression of centrosomal functional genes in multiple myeloma"@en . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Journal of Translational Medicine" . . "Clinical implication of centrosome amplification and expression of centrosomal functional genes in multiple myeloma"@en . "RIV/00216224:14110/13:00065607" . . "Background: Multiple myeloma (MM) is a low proliferative tumor of postgerminal center plasma cell (PC). Centrosome amplification (CA) is supposed to be one of the mechanisms leading to chromosomal instability. Also, CA is associated with deregulation of cell cycle, mitosis, DNA repair and proliferation. The aim of our study was to evaluate the prognostic significance and possible role of CA in pathogenesis and analysis of mitotic genes as mitotic disruption markers. Design and methods: A total of 173 patients were evaluated for this study. CD138+ cells were separated by MACS. Immunofluorescent labeling of centrin was used for evaluation of centrosome amplification in PCs. Interphase FISH with cytoplasmic immunoglobulin light chain staining (cIg FISH) and qRT-PCR were performed on PCs. Results: Based on the immunofluorescent staining results, all patients were divided into two groups: CA positive (38.2%) and CA negative (61.8%)." . . . "RIV/00216224:14110/13:00065607!RIV14-MZ0-14110___" . . "P(GAP304/10/1395), P(NS10207), P(NT11154), P(NT13190)" . "1479-5876" . . "Background: Multiple myeloma (MM) is a low proliferative tumor of postgerminal center plasma cell (PC). Centrosome amplification (CA) is supposed to be one of the mechanisms leading to chromosomal instability. Also, CA is associated with deregulation of cell cycle, mitosis, DNA repair and proliferation. The aim of our study was to evaluate the prognostic significance and possible role of CA in pathogenesis and analysis of mitotic genes as mitotic disruption markers. Design and methods: A total of 173 patients were evaluated for this study. CD138+ cells were separated by MACS. Immunofluorescent labeling of centrin was used for evaluation of centrosome amplification in PCs. Interphase FISH with cytoplasmic immunoglobulin light chain staining (cIg FISH) and qRT-PCR were performed on PCs. Results: Based on the immunofluorescent staining results, all patients were divided into two groups: CA positive (38.2%) and CA negative (61.8%)."@en . "\u0160ev\u010D\u00EDkov\u00E1, Sabina" . . . . . . "77" . "Kubiczkov\u00E1, Lenka" . "[0108BB49CCCC]" . "Dementyeva, Elena" . "000317292200001" . "Kugl\u00EDk, Petr" . . "Kryukov, Fedor" . "Multiple myeloma; Centrosome amplification; Overall survival"@en . "Stefanikova, Zdena" . . "Ihnatov\u00E1, Ivana" . . "H\u00E1jek, Roman" . . . . . . "65696" . "Jarkovsk\u00FD, Ji\u0159\u00ED" . "Minarik, Ji\u0159\u00ED" . "N\u011Bmec, Pavel" . . "10.1186/1479-5876-11-77" . . "11"^^ .