"CZ - \u010Cesk\u00E1 republika" . "14110" . "\u017D\u00E1kov\u00E1, Jana" . . "Crha, Igor" . . "RIV/00216224:14110/11:00052606!RIV12-MZ0-14110___" . . "P(2B08060), P(NS9661), Z(MSM0021622415)" . "2" . . . "4"^^ . "8"^^ . "Zdr\u00E1hal, Zbyn\u011Bk" . . "Seminal plasma proteome in men with azoospermia"@en . "224823" . "Proteom semin\u00E1ln\u00ED plazmy u mu\u017E\u016F s azoospermi\u00ED"@cs . . . "Objective: Seminal plasma is a potential source of biomarkers of many disorders of male reproductive organs. Identification and characterization of individual proteins in seminal plasma could be a useful tool for estimating male infertility. The aim of the study was to analyze proteome differences in men with azoospermia and normospermia. Material and methods: Samples of seminal plasma from men with azoospermia and normospermia were collected and cryopreserved in liquid nitrogen. Detergent CHAPS was used to solubilize the sample proteins. Two dimensional (2-D) gel electrophoresis was used to separate protein isolates. Immobilized gradient pH 3\u201310 NL was used for isoelectric focusation and 12% SDS-PAGE was used for second dimension. Sypro Ruby dye was used to stain the proteins. Software PDQuest was used to analyze quantitative and qualitative differences. Selected proteins were digested by trypsin and identified by MALDI-MS/MS or LC-MS/MS and (using) the Mascot software."@en . . "Bur\u0161\u00EDkov\u00E1, Jana" . "Ventruba, Pavel" . "Pohanka, Michal" . "proteome; male infertility; azoospermia; oxidative stress"@en . . "Kone\u010Dn\u00E1, Hana" . "Proteom semin\u00E1ln\u00ED plazmy u mu\u017E\u016F s azoospermi\u00ED" . "C\u00EDl: Semin\u00E1ln\u00ED plazma obsahuje proteiny, kter\u00E9 jsou d\u016Fle\u017Eit\u00FDmi markery patologi\u00ED reproduk\u010Dn\u00EDho \u00FAstroj\u00ED. C\u00EDlem pr\u00E1ce je analyzovat rozd\u00EDly detekovan\u00E9 v proteomu semin\u00E1ln\u00ED plazmy mu\u017E\u016F s azoospermi\u00ED a d\u00E1rc\u016F spermatu. Materi\u00E1l a metodika: Vzorky semin\u00E1ln\u00ED plazmy byly z\u00EDsk\u00E1ny standardn\u00EDm postupem od mu\u017E\u016F s azoospermi\u00ED a d\u00E1rc\u016F spermatu a zamrazeny v kapaln\u00E9m dus\u00EDku. Proteiny ve vzorc\u00EDch byly solubilizov\u00E1ny lyza\u010Dn\u00EDm pufrem obsahuj\u00EDc\u00EDm detergent CHAPS. V\u00FDsledn\u00E9 proteinov\u00E9 izol\u00E1ty byly separov\u00E1ny dvourozm\u011Brnou gelovou elektrofor\u00E9zou (2-DE). V prvn\u00EDm rozm\u011Bru byl pro izoelektrickou fokusaci pou\u017Eit imobilizovan\u00FD gradient pH 3\u201310 NL, pro d\u011Blen\u00ED v druh\u00E9m rozm\u011Bru 12 % SDS-PAGE. Proteiny v gelu byly barveny fluorescen\u010Dn\u00EDm barvivem Sypro Ruby. Kvalitativn\u00ED a kvantitativn\u00ED rozd\u00EDly mezi jednotliv\u00FDmi vzorky byly vyhodnoceny pomoc\u00ED programu PDQuest. Proteiny ve vybran\u00FDch spotech vykazuj\u00EDc\u00ED rozd\u00EDly byly n\u00E1sledn\u011B \u0161t\u011Bpeny trypsinem a identifikov\u00E1ny pomoc\u00ED MALDI-MS/MS (resp. LC-MS/MS) a vyhodnocovac\u00EDho programu Mascot."@cs . . . . "1211-6645" . . "C\u00EDl: Semin\u00E1ln\u00ED plazma obsahuje proteiny, kter\u00E9 jsou d\u016Fle\u017Eit\u00FDmi markery patologi\u00ED reproduk\u010Dn\u00EDho \u00FAstroj\u00ED. C\u00EDlem pr\u00E1ce je analyzovat rozd\u00EDly detekovan\u00E9 v proteomu semin\u00E1ln\u00ED plazmy mu\u017E\u016F s azoospermi\u00ED a d\u00E1rc\u016F spermatu. Materi\u00E1l a metodika: Vzorky semin\u00E1ln\u00ED plazmy byly z\u00EDsk\u00E1ny standardn\u00EDm postupem od mu\u017E\u016F s azoospermi\u00ED a d\u00E1rc\u016F spermatu a zamrazeny v kapaln\u00E9m dus\u00EDku. Proteiny ve vzorc\u00EDch byly solubilizov\u00E1ny lyza\u010Dn\u00EDm pufrem obsahuj\u00EDc\u00EDm detergent CHAPS. V\u00FDsledn\u00E9 proteinov\u00E9 izol\u00E1ty byly separov\u00E1ny dvourozm\u011Brnou gelovou elektrofor\u00E9zou (2-DE). V prvn\u00EDm rozm\u011Bru byl pro izoelektrickou fokusaci pou\u017Eit imobilizovan\u00FD gradient pH 3\u201310 NL, pro d\u011Blen\u00ED v druh\u00E9m rozm\u011Bru 12 % SDS-PAGE. Proteiny v gelu byly barveny fluorescen\u010Dn\u00EDm barvivem Sypro Ruby. Kvalitativn\u00ED a kvantitativn\u00ED rozd\u00EDly mezi jednotliv\u00FDmi vzorky byly vyhodnoceny pomoc\u00ED programu PDQuest. Proteiny ve vybran\u00FDch spotech vykazuj\u00EDc\u00ED rozd\u00EDly byly n\u00E1sledn\u011B \u0161t\u011Bpeny trypsinem a identifikov\u00E1ny pomoc\u00ED MALDI-MS/MS (resp. LC-MS/MS) a vyhodnocovac\u00EDho programu Mascot." . . "[08976897EFB0]" . "Proteom semin\u00E1ln\u00ED plazmy u mu\u017E\u016F s azoospermi\u00ED"@cs . . . . . . "Proteom semin\u00E1ln\u00ED plazmy u mu\u017E\u016F s azoospermi\u00ED" . "Lousov\u00E1, E." . "Praktick\u00E1 gynekologie" . . . "15" . "RIV/00216224:14110/11:00052606" . "4"^^ . "Seminal plasma proteome in men with azoospermia"@en . .