. . . "K-562 cells; Oxidative stress; Apoptosis; Energy substrates; Hyperglycemia; DNA damage"@en . "1213-8118" . "154" . . "The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. Methods. Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic \u201CDNA comets\u201D assay at 5 mM and 30 mM glucose concentrations. Results. The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant \u2013 N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. Conclusion." . . "6"^^ . "CZ - \u010Cesk\u00E1 republika" . "[92130CA74A75]" . "14110" . "The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. Methods. Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic \u201CDNA comets\u201D assay at 5 mM and 30 mM glucose concentrations. Results. The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant \u2013 N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. Conclusion."@en . "HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS" . . . . "Hruda, Jan" . "Leverve, Xavier" . "261506" . . . "HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS"@en . "RIV/00216224:14110/10:00051723" . . . "000285643800007" . "Biomedical Papers" . "3"^^ . . "RIV/00216224:14110/10:00051723!RIV12-MSM-14110___" . . . . "HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS"@en . . "2"^^ . . "4" . "HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS" . "\u0160r\u00E1mek, Vladim\u00EDr" . "I" .