. . "10"^^ . . . "3"^^ . . "Slaninov\u00E1, Iva" . "14110" . . . "Hammerov\u00E1, Jind\u0159i\u0161ka" . "256127" . . "Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells"@en . "24" . "RIV/00216224:14110/10:00043675" . . . "Gelnarov\u00E1, Eva" . . . "Slunsk\u00E1, Zdenka" . . "Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells"@en . "5"^^ . "Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells" . "T\u00E1borsk\u00E1, Eva" . "0887-2333" . . . . "000276668500001" . "Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells" . "NL - Nizozemsko" . . "benzo(c)phenanthridine alkaloids; sanguilutine; chelilutine; cytotoxicity; oxidative stress; antioxidant"@en . . . "Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their anti-proliferative activities with regard to their ability to induce oxidative stress. We observed potent anti-proliferative activities for both alkaloids against three tumor (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and an unequal sensitivity with regard to individual cell lines but independent of the character of the cell line (i.e. tumor vs. normal). Dihydrofluorescein diacetate staining was used for measurement of intracellular ROS accumulation, not only after sanguilutine and chelilutine, but also after sanguinarine and chelerythrine treatment. In addition the antioxidative effect was studied. The effect of the alkaloids was compared with the effect of commonly used antioxidants such as trolox, caffeine acid, and chlorogenic acid." . "Toxicology in Vitro" . . "3" . "P(GA525/08/0819), P(LC06077), Z(MSM0021622415)" . . . "Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their anti-proliferative activities with regard to their ability to induce oxidative stress. We observed potent anti-proliferative activities for both alkaloids against three tumor (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and an unequal sensitivity with regard to individual cell lines but independent of the character of the cell line (i.e. tumor vs. normal). Dihydrofluorescein diacetate staining was used for measurement of intracellular ROS accumulation, not only after sanguilutine and chelilutine, but also after sanguinarine and chelerythrine treatment. In addition the antioxidative effect was studied. The effect of the alkaloids was compared with the effect of commonly used antioxidants such as trolox, caffeine acid, and chlorogenic acid."@en . . "RIV/00216224:14110/10:00043675!RIV11-GA0-14110___" . "[0233502C3970]" .