. . "RIV/00216224:14110/08:00028239" . . . "4"^^ . "Slab\u00FD, Ond\u0159ej" . "Significance of microRNA-21 in colorectal cancer pathogenesis." . . "FEBS" . . "MicroRNAs (miRNAs) are endogenously expressed short non-coding RNAs, that repress protein translation through binding to target mRNAs. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous studies, mainly based on microarrays technology applied on colorectal cancer cell lines, showed altered expression levels of several miRNAs in colorectal cancer (CRC). In our study, we examined by Real-Time PCR expression levels of microRNA-21 (miR-21) in tumors of 29 colorectal cancer patients. For 6 cases of CRC samples also adjacent non-tumor tissues were analyzed. For data normalization we tried different approaches (18S rRNA, GAPDH, let-7a-1). Finally, variability of let-7a-1 expression was shown to be the lowest." . "1742-464X" . "P(NR9076)" . . . . "2008-01-01+01:00"^^ . "Hrstka, Roman" . "RIV/00216224:14110/08:00028239!RIV10-MZ0-14110___" . . . . . "colorectal; microRNAs"@en . . "Vyzula, Rostislav" . . "Oxford, UK" . "R\u016F\u010Dkov\u00E1, Eva" . "1"^^ . "Significance of microRNA-21 in colorectal cancer pathogenesis."@en . "FEBS Journal" . "Significance of microRNA-21 in colorectal cancer pathogenesis." . . . "Significance of microRNA-21 in colorectal cancer pathogenesis."@en . "[BC18C7BED89B]" . "MicroRNAs (miRNAs) are endogenously expressed short non-coding RNAs, that repress protein translation through binding to target mRNAs. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous studies, mainly based on microarrays technology applied on colorectal cancer cell lines, showed altered expression levels of several miRNAs in colorectal cancer (CRC). In our study, we examined by Real-Time PCR expression levels of microRNA-21 (miR-21) in tumors of 29 colorectal cancer patients. For 6 cases of CRC samples also adjacent non-tumor tissues were analyzed. For data normalization we tried different approaches (18S rRNA, GAPDH, let-7a-1). Finally, variability of let-7a-1 expression was shown to be the lowest."@en . "7"^^ . "394693" . "Ovesn\u00E1, Jaroslava" . "14110" . "Athens" . . "Svoboda, Marek" . "Dubsk\u00E1, Lenka" .