"[3C494365CB76]" . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "000242136000009" . . . . "\u017Daloud\u00EDk, Jan" . . "Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy" . . "Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)\u2013based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications." . "Multiple myeloma; immunotherapy; interferon-gamma; T cell; CFSE"@en . . . . "Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy" . "RIV/00216224:14110/06:00018909!RIV10-MZ0-14110___" . "Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy"@en . "23/2006" . "Medical Oncology" . "7"^^ . . "Kov\u00E1\u0159ov\u00E1, Lucie" . "480511" . . . . "7"^^ . "8"^^ . "Zahradov\u00E1, Lenka" . "O\u010Dadl\u00EDkov\u00E1, Darina" . "14110" . "H\u00E1jek, Roman" . "3" . "Mich\u00E1lek, Jaroslav" . "P(NC7475)" . "Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)\u2013based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications."@en . . . . "Penka, Miroslav" . . . . . "1559-131X" . . "Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy"@en . . "RIV/00216224:14110/06:00018909" .