. . "Neionogenn\u00ED tenzid poly(ethylen glycol) pyrenebutano\u00E1t byl pou\u017Eit p\u0159i CIEF s fluorimetrickou detekc\u00ED. Toto barvivo dynamicky modifikovalo analyzovan\u00E9 \u010D\u00E1stice a umo\u017Enilo tak jejich citlivou detekci a identifikaci." . "\u0160lais, Karel" . "CZ - \u010Cesk\u00E1 republika" . . . "Hork\u00FD, Jaroslav" . "R\u016F\u017Ei\u010Dka, Filip" . . "8"^^ . "Neionogenn\u00ED tenzid poly(ethylen glycol) pyrenebutano\u00E1t byl pou\u017Eit p\u0159i CIEF s fluorimetrickou detekc\u00ED. Toto barvivo dynamicky modifikovalo analyzovan\u00E9 \u010D\u00E1stice a umo\u017Enilo tak jejich citlivou detekci a identifikaci."@cs . "RIV/00216224:14110/06:00016450" . "5"^^ . . . "Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate."@en . . . "Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate."@en . . "78" . "P(GA301/99/0045)" . "Kapil\u00E1rn\u00ED izoelektrick\u00E1 fokusace a fluorimetrick\u00E1 detekce dynamicky modifikovan\u00FDch protein\u016F a mikroorganizm\u016F"@cs . "Hol\u00E1, Veronika" . "Kapil\u00E1rn\u00ED izoelektrick\u00E1 fokusace a fluorimetrick\u00E1 detekce dynamicky modifikovan\u00FDch protein\u016F a mikroorganizm\u016F" . "[8078F934798C]" . "14110" . . "3"^^ . . . . "0003-2700" . . "Hork\u00E1, Marie" . . . "8438-8444" . "Analytical Chemistry" . "Kapil\u00E1rn\u00ED izoelektrick\u00E1 fokusace a fluorimetrick\u00E1 detekce dynamicky modifikovan\u00FDch protein\u016F a mikroorganizm\u016F"@cs . "24" . "481424" . . "Kapil\u00E1rn\u00ED izoelektrick\u00E1 fokusace a fluorimetrick\u00E1 detekce dynamicky modifikovan\u00FDch protein\u016F a mikroorganizm\u016F" . "RIV/00216224:14110/06:00016450!RIV08-GA0-14110___" . "capillary isoelectric focusing and fluorometric; detection; proteins; microorganisms; poly(ethylene glycol) pyrenebutanoate."@en . "The nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. This dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. The values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pI markers and were found comparable with pI of the native compounds. The mixed cultures of proteins and microorganisms, Escherichia coli CCM 3954, Staphylococcus epidermidis CCM 4418, Proteus vulgaris, Enterococcus faecalis CCM 4224, and Stenotrophomonas maltophilia, the strains of the yeast cells, Candida albicans CCM 8180, Candida krusei, Candida parapsilosis, Candida glabrata, Candida tropicalis, and Saccharomyces cerevisiae were reproducibly focused and separated by the suggested technique. Using UV excitation for the on-column fluorometric detection, the minimum detectable amoun"@en . .