. "CIEF protein\u016F a mikroorganizm\u016F v dynamicky modifikovan\u00E9 k\u0159emi\u010Dit\u00E9 kapil\u00E1\u0159e s UV detekc\u00ED." . . . "P(GA301/99/0045)" . "152-159" . "CIEF protein\u016F a mikroorganizm\u016F v dynamicky modifikovan\u00E9 k\u0159emi\u010Dit\u00E9 kapil\u00E1\u0159e s UV detekc\u00ED." . "1570-0232" . "Hork\u00E1, Marie" . "Hol\u00E1, Veronika" . . "14110" . "Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection."@en . . . . . . . "Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection."@en . "841" . "\u0160lais, Karel" . . "R\u016F\u017Ei\u010Dka, Filip" . . "Navr\u017Een\u00E1 CIEF metoda umo\u017E\u0148uje citliv\u011B a reprodukovateln\u011B v gradientu pH 3-10 detekovat a identifikovat proteiny i mikroorganizmy a to jak z \u010Dist\u00FDch kultur, tak ze sm\u011Bs\u00ED."@cs . "468740" . . "capillary isoelectric focusing; proteins; microorganisms; dynamically modified fused silica; UV detection."@en . "5"^^ . "CZ - \u010Cesk\u00E1 republika" . . "We suggest a method for the reproducible and efficient capillary isoelectric focusing of proteins and microorganisms in the pH gradient 3-10. The method involves the segmental injection of the simple ampholytes, the solution of the selected electrolytes, and the sample mixture of bioanalytes and carrier ampholytes to the fused silica capillaries dynamically modified by poly(ethylene glycol), PEG 4000, which is added to the catholyte, the anolyte and injected solutions. In order to receive the reproducible results, the capillaries were rinsed by the mixture of acetone/ethanol between analyses. For the tracing of the pH gradients the low-molecular-mass pI markers were used. The simple proteins and the mixed cultures of microorganisms, Saccharomyces cerevisiae CCM 8191, Escherichia coli CCM 3954, Candida albicans CCM 8180, Candida parapsilosis, Candida krusei, Staphylococcus aureus, Streptococcus agalactiae CCM 6187, Enterococcus faecalis CCM 4224, Staphylococcus epidermidis CCM 4418 and Stenotrophomonas"@en . . . "8"^^ . . "Hork\u00FD, Jaroslav" . "CIEF protein\u016F a mikroorganizm\u016F v dynamicky modifikovan\u00E9 k\u0159emi\u010Dit\u00E9 kapil\u00E1\u0159e s UV detekc\u00ED."@cs . "Navr\u017Een\u00E1 CIEF metoda umo\u017E\u0148uje citliv\u011B a reprodukovateln\u011B v gradientu pH 3-10 detekovat a identifikovat proteiny i mikroorganizmy a to jak z \u010Dist\u00FDch kultur, tak ze sm\u011Bs\u00ED." . "Journal of Chromatography B" . "CIEF protein\u016F a mikroorganizm\u016F v dynamicky modifikovan\u00E9 k\u0159emi\u010Dit\u00E9 kapil\u00E1\u0159e s UV detekc\u00ED."@cs . "RIV/00216224:14110/06:00016449" . . "[77890FF0B653]" . "3"^^ . . "1-2" . "RIV/00216224:14110/06:00016449!RIV08-GA0-14110___" .