. "CIEF v kysel\u00E9m gradientu pH 2-5 umo\u017E\u0148uje separaci a identifikaci mikrob\u016F i ze sm\u011Bsn\u00FDch kultur."@cs . "385" . "Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection."@en . . . "Kapil\u00E1rn\u00ED isoelektrick\u00E1 fokusace mikroorganism\u016F v pH 2-5 v dynamicky modifikovan\u00E9 FS kapil\u00E1\u0159e s UV detekc\u00ED" . "840-846" . "4"^^ . "P(GA301/99/0045)" . . . "Hol\u00E1, Veronika" . "Analytical and Bioanalytical Chemistry" . . . . "3"^^ . "\u0160lais, Karel" . "7"^^ . . . . "CIEF v kysel\u00E9m gradientu pH 2-5 umo\u017E\u0148uje separaci a identifikaci mikrob\u016F i ze sm\u011Bsn\u00FDch kultur." . "Kapil\u00E1rn\u00ED isoelektrick\u00E1 fokusace mikroorganism\u016F v pH 2-5 v dynamicky modifikovan\u00E9 FS kapil\u00E1\u0159e s UV detekc\u00ED"@cs . "Kapil\u00E1rn\u00ED isoelektrick\u00E1 fokusace mikroorganism\u016F v pH 2-5 v dynamicky modifikovan\u00E9 FS kapil\u00E1\u0159e s UV detekc\u00ED" . "RIV/00216224:14110/06:00016448!RIV08-GA0-14110___" . "[EFD5FFCF8844]" . . "The isoelectric points of many microbial cells lie within the pH range spanning from 1.5 to 4.5. In this work, we suggest a CIEF method for the separation of cells according to their isoelectric points in the pH range of 2-5. It includes the segmental injection of the sample pulse composed of the segment of the selected simple ampholytes, the segment of the bioanalytes and the segment of carrier ampholytes into fused silica capillaries dynamically modified by poly(ethylene glycole). This polymer dissolved in the catholyte, in the anolyte and in the injected sample pulse was used for a prevention of the bioanalyte adsorption on the capillary surface and for the reduction of the electroosmotic flow. Between each focusing run, the capillaries were washed with the mixture of acetone/ethanol to achieve the reproducible and efficient CIEF. In order to trace of pH gradients, low-molecular-mass pI markers were used. The mixed cultures of microorganisms, Escherichia coli CCM 3954, Candida albicans CCM 8180, Ca"@en . . . "1618-2642" . "14110" . . "capillary isoelectric focusing; microorganisms; Staphylococcus; Candida; Escherichia coli; UV detection."@en . . "CZ - \u010Cesk\u00E1 republika" . . "Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection."@en . "5" . "R\u016F\u017Ei\u010Dka, Filip" . . . . "RIV/00216224:14110/06:00016448" . "481423" . "Kapil\u00E1rn\u00ED isoelektrick\u00E1 fokusace mikroorganism\u016F v pH 2-5 v dynamicky modifikovan\u00E9 FS kapil\u00E1\u0159e s UV detekc\u00ED"@cs . "Hork\u00E1, Marie" .