. . . "P(GA309/03/1199), Z(MSM0021622404)" . "Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models" . "0272-4340" . . . "Hradilov\u00E1 Sv\u00ED\u017Eensk\u00E1, Ivana" . "Cellular and Molecular Neurobiology" . . "13"^^ . . "Klus\u00E1kov\u00E1, Ilona" . . "RIV/00216224:14110/06:00015796!RIV10-GA0-14110___" . . "5"^^ . . "5"^^ . . "4" . . "Jan\u010D\u00E1lek, Radim" . "Pejchalov\u00E1, Kate\u0159ina" . "1. Several lines of evidence suggest that cytokines and their receptors are initiators of changes in the activity of dorsal root ganglia (DRG) neurons, but their cellular distribution is still very limited or controversial. Therefore, the goal of present study was to investigate immunohistochemical distribution of TNF-a and TNF receptor-1 (TNFR1) proteins in the rat DRG following three types of nerve injury. 2. The unilateral sciatic and spinal nerve ligation as well as the sciatic nerve transection were used to induce changes in the distribution of TNF-a and TNFR1 proteins. The TNF-a and TNFR1 immunofluorescence was assessed in the L4-L5 DRG affected by nerve injury for 1 and 2 weeks, and compared with the contralateral ones and those removed from naive or sham-operated rats. A part of the sections was incubated for simultaneous immunostaining for TNF-a and ED-1. The immunofluorescence brightness was measured by image analysis system (LUCIA-G v4." . "Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models" . . "NL - Nizozemsko" . "Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models"@en . . "Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models"@en . "[CB3372D38C69]" . . "RIV/00216224:14110/06:00015796" . "1. Several lines of evidence suggest that cytokines and their receptors are initiators of changes in the activity of dorsal root ganglia (DRG) neurons, but their cellular distribution is still very limited or controversial. Therefore, the goal of present study was to investigate immunohistochemical distribution of TNF-a and TNF receptor-1 (TNFR1) proteins in the rat DRG following three types of nerve injury. 2. The unilateral sciatic and spinal nerve ligation as well as the sciatic nerve transection were used to induce changes in the distribution of TNF-a and TNFR1 proteins. The TNF-a and TNFR1 immunofluorescence was assessed in the L4-L5 DRG affected by nerve injury for 1 and 2 weeks, and compared with the contralateral ones and those removed from naive or sham-operated rats. A part of the sections was incubated for simultaneous immunostaining for TNF-a and ED-1. The immunofluorescence brightness was measured by image analysis system (LUCIA-G v4."@en . . . "26" . . . "14110" . "Dubov\u00FD, Petr" . . "000242657500011" . "480238" . "proinflammatory cytokines; unilateral nerve injury; bilateral reaction"@en . .