"3" . "Anticancer immunotherapy using dendritic cell-based vaccines is a strategy aimed at the induction and maintenance of immune responses against cancer cells.Clinical applications of dendritic cells (DCs) require stringent adherence to Good Manufacturing Practice (GMP) methods and rigorous standardization of DC-based vaccine preparation. Recently,closed systems for DC culture have been developed with a goal to minimize the risk of contamination.Here,we compare the yield,immunophenotype, and functional properties of DCs generated in Lifecell X-Fold culture bags and in plastic wells, both from adherence-selected monocytes, and review the current literature on closed systems for DC generation. We found that both the overall yield and the yield of CD83+ cells in cell culture bags was lower than in the standard culture method. No statistically significant differences were observed in the expression of DC immunophenotypic markers. The capability of DCs cultured in bags and in wells to induce the proliferation" . "Han\u00E1k, Libor" . "dendritic cells; bag culture; closed system; cellular vaccine; immunotherapy"@en . . . "P\u0159\u00EDprava dendritick\u00FDch bun\u011Bk s pou\u017Eit\u00EDm kultiva\u010Dn\u00EDch vak\u016F - popis metody a shrnut\u00ED literatury."@cs . "1024-5332" . "565291" . . . "9"^^ . "14110" . . "B\u00FCchler, Tom\u00E1\u0161" . "RIV/00216224:14110/04:00012305!RIV09-MZ0-14110___" . "Generation of Dendritic Cells Using Cell Culture Bags - Description of a Method and Review of Literature." . . . "7"^^ . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Musilov\u00E1, Romana" . "RIV/00216224:14110/04:00012305" . "9"^^ . "Generation of Dendritic Cells Using Cell Culture Bags - Description of a Method and Review of Literature." . . "Bourkov\u00E1, Ludmila" . "Generation of Dendritic Cells Using Cell Culture Bags - Description of a Method and Review of Literature."@en . "Kov\u00E1\u0159ov\u00E1, Lucie" . "Generation of Dendritic Cells Using Cell Culture Bags - Description of a Method and Review of Literature."@en . "Mich\u00E1lek, Jaroslav" . "P\u0159\u00EDprava dendritick\u00FDch bun\u011Bk s pou\u017Eit\u00EDm kultiva\u010Dn\u00EDch vak\u016F - popis metody a shrnut\u00ED literatury."@cs . "[CF1C4B3D3E2B]" . . . . "H\u00E1jek, Roman" . . "Anticancer immunotherapy using dendritic cell-based vaccines is a strategy aimed at the induction and maintenance of immune responses against cancer cells.Clinical applications of dendritic cells (DCs) require stringent adherence to Good Manufacturing Practice (GMP) methods and rigorous standardization of DC-based vaccine preparation. Recently,closed systems for DC culture have been developed with a goal to minimize the risk of contamination.Here,we compare the yield,immunophenotype, and functional properties of DCs generated in Lifecell X-Fold culture bags and in plastic wells, both from adherence-selected monocytes, and review the current literature on closed systems for DC generation. We found that both the overall yield and the yield of CD83+ cells in cell culture bags was lower than in the standard culture method. No statistically significant differences were observed in the expression of DC immunophenotypic markers. The capability of DCs cultured in bags and in wells to induce the proliferation"@en . "P(NC7475)" . . "Protin\u00E1dorov\u00E1 terapie pou\u017E\u00EDvaj\u00EDc\u00ED vakc\u00EDny na b\u00E1zi dendritick\u00FDch bun\u011Bk je strategie sm\u011B\u0159ovan\u00E1 na vyvol\u00E1n\u00ED a udr\u017Een\u00ED imunitn\u00EDch odpov\u011Bd\u00ED v\u016F\u010Di n\u00E1dorov\u00FDm bu\u0148k\u00E1m. Klinick\u00E9 aplikace dendritick\u00FDch bun\u011Bk (DB) vy\u017Eaduj\u00ED p\u0159\u00EDsn\u00E9 dodr\u017Eov\u00E1n\u00ED postup\u016F Spr\u00E1vn\u00E9 v\u00FDrobn\u00ED praxe (GMP) a standardizace p\u0159\u00EDpravy vakc\u00EDn zalo\u017Een\u00FDch na DB. V posledn\u00ED dob\u011B byly vyvinuty uzav\u0159en\u00E9 syst\u00E9my pro DB kultivaci s c\u00EDlem minimalizovat riziko kontaminace. V t\u00E9to pr\u00E1ciporovn\u00E1v\u00E1me v\u00FDt\u011B\u017Eek, imunofenotyp a funk\u010Dn\u00ED vlastnosti DB p\u0159ipraven\u00FDch z adherentn\u00EDch monocyt\u016F v Lifecell X-Fold kultiva\u010Dn\u00EDch vac\u00EDch a um\u011Blohmotn\u00FDch desti\u010Dk\u00E1ch, a pod\u00E1v\u00E1me p\u0159ehled sou\u010Dasn\u00E9 literatury o p\u0159\u00EDprav\u011B DB v uzav\u0159en\u00FDch syst\u00E9mech. Zjistili jsme, \u017Ee celkov\u00FD v\u00FDt\u011B\u017Eek a v\u00FDt\u011B\u017Eek CD83+ bun\u011Bk v kultiva\u010Dn\u00EDch vac\u00EDch byl ni\u017E\u0161\u00ED ne\u017E p\u0159i klasick\u00E9 kultivaci.Nebyly pozorov\u00E1ny \u017E\u00E1dn\u00E9 statisticky v\u00FDznamn\u00E9 rozd\u00EDly v expresi DB imunofenotypick\u00FDch marker\u016F. Schopnosti DB p\u0159ipraven\u00FDch v kultiva\u010Dn\u00EDch vac\u00EDch nebo desti\u010Dk\u00E1ch vyvolat proliferaci allogenn\u00EDch bun\u011Bk byly shodn\u00E9. V\u00FDkonnost DB ve sm\u00ED\u0161e"@cs . . . . . "9" . . "Bulikov\u00E1, Alena" . "O\u010Dadl\u00EDkov\u00E1, Darina" . . . . "Hematology" . . . . .