"2004-09-24+02:00"^^ . . "Tarabov\u00E1, Bohuslava" . "Montpellier, Francie" . . . "14110" . "Effect of Sigma Ligand Haloperidol on Cardiac Excitability"@en . . . "\u00DA\u010Dinek sigma ligandu haloperidolu na srde\u010Dn\u00ED excitabilitu"@cs . . "La Grande-Motte" . . . "Kri\u017Eanov\u00E1, Olga" . "562004" . "INSERM" . "4"^^ . "Effect of Sigma Ligand Haloperidol on Cardiac Excitability" . "New Frontiers in Basic Cardiovascular Research" . . "cardiac excitability; sigma receptor; haloperidol; ionic currents; inhibition"@en . "Nov\u00E1kov\u00E1, Marie" . "Sigma receptor ligand haloperidol je psychotropn\u00ED l\u00E1tka u\u017E\u00EDvan\u00E1 v l\u00E9\u010Db\u011B r\u016Fzn\u00FDch psychiatrick\u00FDch onemocn\u011Bn\u00ED. Byly pops\u00E1ny t\u011B\u017Ek\u00E9 vedlej\u0161\u00ED \u00FA\u010Dinky na kardiovaskul\u00E1rn\u00ED syst\u00E9m (hlavn\u011B komorov\u00E9 arythmie). zkoumali jsme \u00FA\u010Dinek 10 microM haloperidolu na srde\u010Dn\u00ED iontov\u00E9 proudy u dvou model\u016F -izolovan\u00FDch kardiomyocyt\u016F potkana a HEK293 bun\u011Bk s p\u0159echodn\u011B exprimovan\u00FDm CaV1.2 L-typem kalciov\u00E9ho kan\u00E1lu. Exprese sigma receptor\u016F v obou modelech byla m\u011B\u0159ena RT-PCR. Primery were z\u00EDsk\u00E1ny ze sekvence sigma receptor\u016F (potkan-Genbank number 38541100, lidsk\u00E9-Genbank number 22212933) a amplifikov\u00E1ny 185bp fragmentem v pozici 499 - 684 nt. Metodou whole cell patch-clamp jsme zji\u0161\u0165ovali \u00FA\u010Dinek haloperidolu na sod\u00EDkov\u00E1, drasl\u00EDkov\u00E9 a kalciov\u00FD proudy. V kardiomyocytech potkana haloperidol inhiboval 95% INa aktivovan\u00E9ho 40 ms nap\u011B\u0165ov\u00FDmi pulzy z 75 mV do 20 mV. Inhibice Ito a IK,end byla m\u011B\u0159ena 300 ms nap\u011B\u0165ov\u00FDmi pulzy z 75 mV do +40 mV. Byla pozorov\u00E1na 80% inhibice Ito a 37% inhibice IK,end, v\u017Edy reverzibiln\u00ED. Inaktivace Ito byla hal"@cs . "[AA6058FC10CF]" . . "1"^^ . "7"^^ . . "33" . . "Matejovi\u010D, Peter" . "RIV/00216224:14110/04:00011578" . "Effect of Sigma Ligand Haloperidol on Cardiac Excitability"@en . "RIV/00216224:14110/04:00011578!RIV08-GA0-14110___" . "P(GA305/04/1385)" . . . "Sigma receptor ligand haloperidol is a psychotropic drug used in the treatment of various psychiatric disorders. Severe cardiovascular side effects (mostly ventricular arrhythmias) have been often reported as a consequence of this treatment. Thus, we have investigated the effects of 10 \u013EM haloperidol on cardiac ionic currents in two models freshly isolated rat ventricular cardiomyocytes and in HEK 293 cells with transiently expressed CaV1.2 L-type calcium channel. Gene expression of sigma receptors in both models was measured by RT-PCR. Primers were designed from sequence of sigma receptors (for rat -Genbank number 38541100, for human Genbank number 22212933) and amplified 185bp fragment in the position 499 - 684 nt. The whole cell patch-clamp method has been employed to assess the effect of haloperidol on sodium, potassium and calcium currents. In rat cardiac cells, haloperidol inhibited 95% of INa activated by 40 ms voltage pulses from 75 mV to 20 mV. The inhibition of Ito and IK,end were measured b"@en . . "B\u00E9barov\u00E1, Mark\u00E9ta" . . "\u00DA\u010Dinek sigma ligandu haloperidolu na srde\u010Dn\u00ED excitabilitu"@cs . . "Effect of Sigma Ligand Haloperidol on Cardiac Excitability" . . "Sigma receptor ligand haloperidol is a psychotropic drug used in the treatment of various psychiatric disorders. Severe cardiovascular side effects (mostly ventricular arrhythmias) have been often reported as a consequence of this treatment. Thus, we have investigated the effects of 10 \u013EM haloperidol on cardiac ionic currents in two models freshly isolated rat ventricular cardiomyocytes and in HEK 293 cells with transiently expressed CaV1.2 L-type calcium channel. Gene expression of sigma receptors in both models was measured by RT-PCR. Primers were designed from sequence of sigma receptors (for rat -Genbank number 38541100, for human Genbank number 22212933) and amplified 185bp fragment in the position 499 - 684 nt. The whole cell patch-clamp method has been employed to assess the effect of haloperidol on sodium, potassium and calcium currents. In rat cardiac cells, haloperidol inhibited 95% of INa activated by 40 ms voltage pulses from 75 mV to 20 mV. The inhibition of Ito and IK,end were measured b" . . . "Lacinov\u00E1, Lubica" . "P\u00E1sek, Michal" . .