. "561910" . "Effect of haloperidol on voltage dependent cardiac ion channels" . "Smolenice" . "\u00DA\u010Dinek haloperidolu na nap\u011B\u0165ov\u011B z\u00E1visl\u00E9 iontov\u00E9 proudy srdce"@cs . "Membrane Channels, Transporters and Receptors" . "\u00DA\u010Dinek haloperidolu na nap\u011B\u0165ov\u011B z\u00E1visl\u00E9 iontov\u00E9 proudy srdce"@cs . "Nov\u00E1kov\u00E1, Marie" . "RIV/00216224:14110/04:00011470!RIV08-GA0-14110___" . . "Haloperidol je neuroleptikum \u00FA\u010Dinn\u00E9 v l\u00E9\u010Db\u011B r\u016Fzn\u00FDch psychiatrick\u00FDch onemocn\u011Bn\u00ED. Tato l\u00E9\u010Dba \u010Dasto vyvol\u00E1v\u00E1 srde\u010Dn\u00ED arytmie. Metodou whole cell patch-clamp jsme zkoumali vliv haloperidolu na srde\u010Dn\u00ED sod\u00EDkov\u00FD, drasl\u00EDkov\u00E9 a v\u00E1pn\u00EDkov\u00FD proudy. Byla pou\u017Eita 10 microM koncentrace haloperidolu. U kardiomyocyt\u016F izolovan\u00FDch z dosp\u011Bl\u00FDch srde\u010Dn\u00EDch komor potkana haloperidol inhiboval 95% sod\u00EDkov\u00E9ho proudu (INa) aktivovan\u00E9ho 40 ms nap\u011B\u0165ov\u00FDmi pulzy z -75 mV do -20 mV. Blok\u00E1da p\u0159echodn\u00E9ho proudu z bu\u0148ky (Ito) a proudu na konci pulzu (IK,end) byly m\u011B\u0159eny 300 ms nap\u011B\u0165ov\u00FDmi pulzy z -75 mV do +40 mV. Haloperidol zp\u016Fsobil 80% blok\u00E1du Ito a 37% blok\u00E1du IK,end, v\u017Edy reverzibiln\u00ED. D\u00E1le jsme zkoumali vliv haloperidolu na CaV1.2 L-typ kalciov\u00E9ho kan\u00E1lu p\u0159echodn\u011B exprimovan\u00FD v HEK 293 bu\u0148k\u00E1ch. Haloperidol inhiboval zhruba 58% amplitudy m\u011B\u0159en\u00E9 na vrcholu IV k\u0159ivky. Inhibice byla reverzibiln\u00ED a nap\u011B\u0165ov\u011B z\u00E1visl\u00E1. Zv\u00FD\u0161en\u00ED amplitudy depolariza\u010Dn\u00EDch pulz\u016F siln\u011B zv\u00FD\u0161ilo velikost ihnibice proudu."@cs . "Bratislava" . "Effect of haloperidol on voltage dependent cardiac ion channels"@en . "P(GA305/04/1385)" . . . . "B\u00E9barov\u00E1, Mark\u00E9ta" . "Lacinov\u00E1, Lubica" . "[A2E9F3438061]" . "Effect of haloperidol on voltage dependent cardiac ion channels" . "RIV/00216224:14110/04:00011470" . "Matejovi\u010D, Peter" . . . . . "2004-06-05+02:00"^^ . "haloperidol; patch clamp; rat cardiomyocytes; HEK 293 cells"@en . . . . "4"^^ . "Haloperidol is a neuroleptic agent effective in treatment of various psychiatric diseases. Cardiac arrhythmias have been associated with this therapy. We have investigated effects of haloperidol on cardiac sodium, potassium and calcium channels using whole cell patch-clamp method. 10 microM concentration of haloperidol was tested. In cardiomyocytes isolated from adult rat ventricles, haloperidol inhibited 95% of sodium current (INa) activated by 40 ms voltage pulses from -75 mV to -20 mV. The block of the transient outward potassium current (Ito) and current at the end of the pulse (IK,end) were measured by 300 ms voltage pulses from -75 mV to +40 mV. Haloperidol caused 80% block of Ito and 37% block of IK,end. In all cases the block was reversible. Further, we have investigated CaV1.2 L-type calcium channel transiently expressed in HEK 293 cells. Haloperidol inhibited approximately 58% of inward current amplitude measured in peak of IV relation. The inhibition was reversible and voltage dependent. In"@en . . . "P\u00E1sek, Michal" . "Haloperidol is a neuroleptic agent effective in treatment of various psychiatric diseases. Cardiac arrhythmias have been associated with this therapy. We have investigated effects of haloperidol on cardiac sodium, potassium and calcium channels using whole cell patch-clamp method. 10 microM concentration of haloperidol was tested. In cardiomyocytes isolated from adult rat ventricles, haloperidol inhibited 95% of sodium current (INa) activated by 40 ms voltage pulses from -75 mV to -20 mV. The block of the transient outward potassium current (Ito) and current at the end of the pulse (IK,end) were measured by 300 ms voltage pulses from -75 mV to +40 mV. Haloperidol caused 80% block of Ito and 37% block of IK,end. In all cases the block was reversible. Further, we have investigated CaV1.2 L-type calcium channel transiently expressed in HEK 293 cells. Haloperidol inhibited approximately 58% of inward current amplitude measured in peak of IV relation. The inhibition was reversible and voltage dependent. In" . . "54" . "Tarabov\u00E1, Bohuslava" . . . . "6"^^ . . "14110" . "1"^^ . "Institute of Molecular Physiology and Genetics" . "Effect of haloperidol on voltage dependent cardiac ion channels"@en . .