"2"^^ . "DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) isolated from rabbit testes 10 days post infection. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the transcript levels generally corresponded to the relative protein concentrations. Genes with high transcript concentrations included those encoding flagellar filament and cytoplasmic filament proteins, prominent lipoproteins and membrane proteins, chaperonins, proteins involved in red-ox balance, chemotaxis regulatory proteins, a V-ATPase operon, and certain metabolic enzymes such as gl"@en . "4"^^ . "\u0160majs, David" . "transcriptome, treponema pallidum"@en . "[B259D801CAD3]" . "Norris, S. J." . . . "Byla studov\u00E1na \u00FArove\u0148 genov\u00E9 exprese u spirochety Treponema pallidum subsp. pallidum (kmen Nichols) kultivovan\u00E9 v podm\u00EDnk\u00E1ch in vivo v kr\u00E1li\u010D\u00EDch varlatech. Spirochety byly izolov\u00E1ny 10 dn\u00ED po inokulaci a pot\u00E9 z nich byla izolov\u00E1na chromosom\u00E1ln\u00ED DNA a celkov\u00E1 RNA. DNA a RNA byla - po ozna\u010Den\u00ED flourescen\u010Dn\u00EDmi barvivy - hybridizov\u00E1na na DNA \u010Dipech, kter\u00E9 obsahuj\u00ED PCR produkty v\u0161ech 1039 predikovan\u00FDch gen\u016F T. pallidum. Nejsiln\u011Bji transkribovan\u00E9 geny (geny s nejvy\u0161\u0161\u00EDm pom\u011Brem mezi zna\u010Denou RNA a DNA) odpov\u00EDdaly protein\u016Fm s nejv\u00FDrazn\u011Bj\u0161\u00EDm sign\u00E1lem identifikovan\u00FDm na dvourozm\u011Brn\u00E9 gelov\u00E9 elektrofor\u00E9ze. Tyto geny k\u00F3duj\u00ED flagel\u00E1rn\u00ED a cyplasmatick\u00E9 filament\u00E1rn\u00ED proteiny, lipoproteiny a membr\u00E1nov\u00E9 antigeny, chaperoniny, proteiny treponem\u00E1ln\u00EDho redox syst\u00E9mu, chemotaktick\u00E9 regula\u010Dn\u00ED proteiny, komponenty V-ATP\u00E1zov\u00E9ho syst\u00E9mu a n\u011Bkter\u00E9 enzymy (nap\u0159. glykolytick\u00E9 dr\u00E1hy). V\u00FDsledky z\u00EDskan\u00E9 pomoc\u00ED DNA \u010Dip\u016F byly nez\u00E1visle potvrzeny pomoc\u00ED PCR anal\u00FDzy v re\u00E1ln\u00E9m \u010Dase. Stanoven\u00ED genov\u00E9 exprese touto metodou u 84 gen\u016F vykaz" . . "Byla studov\u00E1na \u00FArove\u0148 genov\u00E9 exprese u spirochety Treponema pallidum subsp. pallidum (kmen Nichols) kultivovan\u00E9 v podm\u00EDnk\u00E1ch in vivo v kr\u00E1li\u010D\u00EDch varlatech. Spirochety byly izolov\u00E1ny 10 dn\u00ED po inokulaci a pot\u00E9 z nich byla izolov\u00E1na chromosom\u00E1ln\u00ED DNA a celkov\u00E1 RNA. DNA a RNA byla - po ozna\u010Den\u00ED flourescen\u010Dn\u00EDmi barvivy - hybridizov\u00E1na na DNA \u010Dipech, kter\u00E9 obsahuj\u00ED PCR produkty v\u0161ech 1039 predikovan\u00FDch gen\u016F T. pallidum. Nejsiln\u011Bji transkribovan\u00E9 geny (geny s nejvy\u0161\u0161\u00EDm pom\u011Brem mezi zna\u010Denou RNA a DNA) odpov\u00EDdaly protein\u016Fm s nejv\u00FDrazn\u011Bj\u0161\u00EDm sign\u00E1lem identifikovan\u00FDm na dvourozm\u011Brn\u00E9 gelov\u00E9 elektrofor\u00E9ze. Tyto geny k\u00F3duj\u00ED flagel\u00E1rn\u00ED a cyplasmatick\u00E9 filament\u00E1rn\u00ED proteiny, lipoproteiny a membr\u00E1nov\u00E9 antigeny, chaperoniny, proteiny treponem\u00E1ln\u00EDho redox syst\u00E9mu, chemotaktick\u00E9 regula\u010Dn\u00ED proteiny, komponenty V-ATP\u00E1zov\u00E9ho syst\u00E9mu a n\u011Bkter\u00E9 enzymy (nap\u0159. glykolytick\u00E9 dr\u00E1hy). V\u00FDsledky z\u00EDskan\u00E9 pomoc\u00ED DNA \u010Dip\u016F byly nez\u00E1visle potvrzeny pomoc\u00ED PCR anal\u00FDzy v re\u00E1ln\u00E9m \u010Dase. Stanoven\u00ED genov\u00E9 exprese touto metodou u 84 gen\u016F vykaz"@cs . . "Masarykova univerzita. L\u00E9ka\u0159sk\u00E1 fakulta" . . "RIV/00216224:14110/03:00012309!RIV/2005/MZ0/141105/N" . . . "Brno, Komensk\u00E9ho n\u00E1m. 2" . . "Transkriptome of Treponema pallidum: gene expression levels during experimental rabbit infection"@en . "Transkriptom spirochety Treponema pallidum: \u00FArove\u0148 genov\u00E9 exprese b\u011Bhem experiment\u00E1ln\u00ED infekce kr\u00E1l\u00EDk\u016F"@cs . "Sborn\u00EDk abstrakt\u016F XII. konference mlad\u00FDch mikrobiolog\u016F Tom\u00E1\u0161kovy dny 2003" . "14110" . "14 - 15" . "Weinstock, G. M." . . "RIV/00216224:14110/03:00012309" . . "Transkriptom spirochety Treponema pallidum: \u00FArove\u0148 genov\u00E9 exprese b\u011Bhem experiment\u00E1ln\u00ED infekce kr\u00E1l\u00EDk\u016F" . "Transkriptom spirochety Treponema pallidum: \u00FArove\u0148 genov\u00E9 exprese b\u011Bhem experiment\u00E1ln\u00ED infekce kr\u00E1l\u00EDk\u016F"@cs . "Brno" . . . "1"^^ . . . "Transkriptom spirochety Treponema pallidum: \u00FArove\u0148 genov\u00E9 exprese b\u011Bhem experiment\u00E1ln\u00ED infekce kr\u00E1l\u00EDk\u016F" . "631364" . "Mat\u011Bjkov\u00E1, Petra" . . "P(NI7351)" . "Transkriptome of Treponema pallidum: gene expression levels during experimental rabbit infection"@en . . "2003-06-04+02:00"^^ .