"Proceeding of 15th International Conference %22Chromatographic Methods and Human Health%22" . "[26CD2276F98E]" . "Pie\u0161\u0165any" . . "RIV/00216224:14110/03:00008403!RIV09-GA0-14110___" . "Glatz, Zden\u011Bk" . . . "2"^^ . "Determination of malondialdehyde in biological samples by HPLC" . "603365" . . "2"^^ . "DETERMINATION OF MALONDIALDEHYDE IN BIOLOGICAL SAMPLES BY HPLC"@en . "1335-5236" . "DETERMINATION OF MALONDIALDEHYDE IN BIOLOGICAL SAMPLES BY HPLC"@en . "Slovensk\u00E1 chemick\u00E1 spolo\u010Dnos\u0165 pri SAV" . "Determination of malondialdehyde in biological samples by HPLC"@en . "Bratislava" . . "DETERMINATION OF MALONDIALDEHYDE IN BIOLOGICAL SAMPLES BY HPLC" . . . . . "Tomandl, Josef" . "14110" . "Malondialdehyd (MDA) je pro \u017Eiv\u00E9 syst\u00E9my vysoce toxickou l\u00E1tkou. Vznik\u00E1 jako sekund\u00E1rn\u00ED produkt p\u0159i peroxidaci polynenasycen\u00FDch mastn\u00FDch kyselin. Jeho mno\u017Estv\u00ED odr\u00E1\u017E\u00ED intenzitu neenzymov\u00E9 peroxidace lipid\u016F, prob\u00EDhaj\u00EDc\u00ED radik\u00E1lovou reakc\u00ED iniciovanou reaktivn\u00EDmi formami kysl\u00EDku, ale i m\u00EDru aktivace enzymov\u00E9 peroxidace, prob\u00EDhaj\u00EDc\u00ED p\u0159i biosynt\u00E9ze ikosanoid\u016F. Ve vodn\u00E9m prost\u0159ed\u00ED se MDA vyskytuje v r\u016Fzn\u00FDch form\u00E1ch v z\u00E1vislosti na hodnot\u011B pH, v biologick\u00FDch vzorc\u00EDch je zastoupen jako voln\u00FD nebo v\u00E1zan\u00FD na proteiny. MDA lze stanovit p\u0159\u00EDmo nebo po jeho derivatizaci, nejb\u011B\u017En\u011Bji s thiobarbiturovou kyselinou (TBA) za vzniku komplexu MDA(TBA)2 (viz obr.), kter\u00FD lze detekovat fotometricky p\u0159i 532 nm nebo spektrofluorimetricky. V komplexn\u00ED matrici, jakou je nap\u0159. plazma, se v\u0161ak vyskytuje nebo b\u011Bhem vlastn\u00ED derivatizace vzorku vznik\u00E1 cel\u00E1 \u0159ada interferuj\u00EDc\u00EDch l\u00E1tek. Proto ji\u017E byla pro zv\u00FD\u0161en\u00ED specifi\u010Dnosti i senzitivity stanoven\u00ED vyvinuta \u0159ada metodik a technik eliminuj\u00EDc\u00ED tyto interferuj\u00EDc\u00ED l\u00E1tky. Nicm\u00E9n\u011B, z\u00EDskan\u00E9"@cs . . . . "P(GA203/02/1447), Z(MSM 141100004)" . "RIV/00216224:14110/03:00008403" . . . . . "2003-11-10+01:00"^^ . "Malondialdehyde HPLC"@en . "Stanoven\u00ED malondialdehydu v biologick\u00FDch vzorc\u00EDch pomoc\u00ED kapalinov\u00E9 chromatografie"@cs . "1"^^ . "DETERMINATION OF MALONDIALDEHYDE IN BIOLOGICAL SAMPLES BY HPLC" . "Stanoven\u00ED malondialdehydu v biologick\u00FDch vzorc\u00EDch pomoc\u00ED kapalinov\u00E9 chromatografie"@cs . .