"3"^^ . . "Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve" . "2002-01-01+01:00"^^ . . "80-210-2998-6" . "Axotomy induces development of a microenvironment supporting axonal regeneration in the peripheral nervous system. The promotion of axonal regeneration is related with up-regulation of many molecules including neurotrophins in reactive Schwann cells. The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the sensory and motor neurons for axon regeneration, we hypothesize different cellular and extracellular conditions alongside afferent and motor axons. We have optimized indirect immunofluorescence method for quantitative investigation of neurotrophins (BDNF, NGF and NT-3). The cryostat sections (8 \u013Em) through the intact and 14-day-rhizotomized dorsal and ventral roots as well as intact and 14-day-transected femoral nerves were cut and incubated simultaneously. An intensity of immunofluorescence staining expressed was compared using the computer-assisted image analysis (Lucia-G v 4.21). Immunofluorescence"@en . "14110" . "Klus\u00E1kov\u00E1, Ilona" . . . "RIV/00216224:14110/02:00006844!RIV09-GA0-14110___" . "1"^^ . "Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve"@cs . . . "Hradilov\u00E1 Sv\u00ED\u017Eensk\u00E1, Ivana" . . "Dubov\u00FD, Petr" . . "661310" . . "Brno" . "RIV/00216224:14110/02:00006844" . . . "Progress in Basic, Applied, and Diagnostic Histochemistry" . "Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve"@en . . . "Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve"@en . . "Axotomy induces development of a microenvironment supporting axonal regeneration in the peripheral nervous system. The promotion of axonal regeneration is related with up-regulation of many molecules including neurotrophins in reactive Schwann cells. The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the sensory and motor neurons for axon regeneration, we hypothesize different cellular and extracellular conditions alongside afferent and motor axons. We have optimized indirect immunofluorescence method for quantitative investigation of neurotrophins (BDNF, NGF and NT-3). The cryostat sections (8 \u013Em) through the intact and 14-day-rhizotomized dorsal and ventral roots as well as intact and 14-day-transected femoral nerves were cut and incubated simultaneously. An intensity of immunofluorescence staining expressed was compared using the computer-assisted image analysis (Lucia-G v 4.21). Immunofluorescence"@cs . . . "neurotrophins; immunofluorescence; image analysis"@en . "Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve" . "Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve"@cs . "3"^^ . . . "[FC0B4E74301E]" . "Axotomy induces development of a microenvironment supporting axonal regeneration in the peripheral nervous system. The promotion of axonal regeneration is related with up-regulation of many molecules including neurotrophins in reactive Schwann cells. The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the sensory and motor neurons for axon regeneration, we hypothesize different cellular and extracellular conditions alongside afferent and motor axons. We have optimized indirect immunofluorescence method for quantitative investigation of neurotrophins (BDNF, NGF and NT-3). The cryostat sections (8 \u013Em) through the intact and 14-day-rhizotomized dorsal and ventral roots as well as intact and 14-day-transected femoral nerves were cut and incubated simultaneously. An intensity of immunofluorescence staining expressed was compared using the computer-assisted image analysis (Lucia-G v 4.21). Immunofluorescence" . "P(GA309/00/0407), Z(MSM 141100001)" . . . "2002, Brno" . "Masarykova univerzita v Brn\u011B" .