"29.-31.5.2002 Smolenice, Slovensko" . "Sborn\u00EDk XXX th Annual Conference on yeasts" . "664539" . . "Spectrin-like protein in yeasts"@cs . "3"^^ . . . "Spectrin-like protein in yeasts" . "Bratislava" . . . "3"^^ . "RIV/00216224:14110/02:00006734" . . . "Spectrin is the principal component of a system of structural proteins that are associated with the plasma membrane cytoplasmic surface. Its structure and function has been best characterized in mammalian erythrocytes (1). Proteins related to the a- and b-erythrocyte spectrin have been detected in almost all vertebrate tissues, and also in plants (2) and hyphal fungae (3). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies was used for spectrin immunodetection. Band at 220 kDa in cells and protoplasts extracts of both yeast strains was detected. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast speci"@cs . "14110" . "36" . "Spectrin is the principal component of a system of structural proteins that are associated with the plasma membrane cytoplasmic surface. Its structure and function has been best characterized in mammalian erythrocytes (1). Proteins related to the a- and b-erythrocyte spectrin have been detected in almost all vertebrate tissues, and also in plants (2) and hyphal fungae (3). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies was used for spectrin immunodetection. Band at 220 kDa in cells and protoplasts extracts of both yeast strains was detected. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast speci" . . "Spectrin-like protein in yeasts"@en . "Slaninov\u00E1, Iva" . "Spectrin-like protein in yeasts"@en . "RIV/00216224:14110/02:00006734!RIV08-GA0-14110___" . "actin; spectrin; yeast; cytoskeleton"@en . . . "Spectrin is the principal component of a system of structural proteins that are associated with the plasma membrane cytoplasmic surface. Its structure and function has been best characterized in mammalian erythrocytes (1). Proteins related to the a- and b-erythrocyte spectrin have been detected in almost all vertebrate tissues, and also in plants (2) and hyphal fungae (3). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies was used for spectrin immunodetection. Band at 220 kDa in cells and protoplasts extracts of both yeast strains was detected. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast speci"@en . . "Spectrin-like protein in yeasts" . "Spectrin-like protein in yeasts"@cs . "[2FB95E9A60B7]" . . . . "1"^^ . . "Holub\u00E1\u0159ov\u00E1, Alena" . "Svoboda, Augustin" . . "2002-05-29+02:00"^^ . "P(GA204/00/0394), P(GA204/99/D025)" . "\u010Ceskoslovensk\u00E1 mikrobiologick\u00E1 spole\u010Dnost" . . . . .