"CZ - \u010Cesk\u00E1 republika" . . "6" . "The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heat shock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C for up to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37 C. It is sug" . . . . "Svoboda, Augustin" . "RIV/00216224:14110/00:00005108!RIV/2002/GA0/141102/N" . . "The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heat shock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C for up to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37 C. It is sug"@en . "381" . "73" . "703691" . "11"^^ . "Saccharomyces cerevisiae, heat shock, Hsp104, actin, microtubules"@en . . . "Scripta medica" . "Holub\u00E1\u0159ov\u00E1, Alena" . . "A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures" . . . "A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures" . "[0321594EAD07]" . . "1211-3395" . . "A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures"@en . "3"^^ . . "0"^^ . . "RIV/00216224:14110/00:00005108" . . "0"^^ . "A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures"@en . "M\u00FCller, Petr" . "3"^^ . "14110" . . "P(GA204/00/0394)" . . . .