. . "benzophenenthridine alkaloids, cytotoxicity, cytoskeleton,"@en . . . "Vliv alkaloid\u016F Chelidonium majus na n\u00E1dorov\u00E9 bu\u0148ky a kvasinky" . . "The effect of Chelidonius majus alkaloids on tumor and yeast cells"@en . "1"^^ . "RIV/00216224:14110/00:00003448!RIV/2002/GA0/141102/N" . . . . . . "Cells II" . "KOPP Publishing" . "4"^^ . . "Vliv alkaloid\u016F Chelidonium majus na n\u00E1dorov\u00E9 bu\u0148ky a kvasinky"@cs . "[1846AE7025F9]" . "Slaninov\u00E1, Iva" . "RIV/00216224:14110/00:00003448" . "730932" . "Bocho\u0159\u00E1kov\u00E1, Hana" . . "Vliv alkaloid\u016F Chelidonium majus na n\u00E1dorov\u00E9 bu\u0148ky a kvasinky" . . "C\u011Bsk\u00E9 Bud\u011Bjovice" . "Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect" . "C\u011Bsk\u00E9 Bud\u011Bjovice" . "T\u00E1borsk\u00E1, Eva" . "0"^^ . "4"^^ . . "Slanina, Ji\u0159\u00ED" . "The effect of Chelidonius majus alkaloids on tumor and yeast cells"@en . "14110" . "0"^^ . "2000-01-01+01:00"^^ . . "Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect"@en . "P(GA204/99/D025)" . . . . "Vliv alkaloid\u016F Chelidonium majus na n\u00E1dorov\u00E9 bu\u0148ky a kvasinky"@cs . "97" . .