"P(GBP501/12/G055), S" . . . "A new setup for direct microspectroscopic monitoring of singlet oxygen (O-1(2)) has been developed in our laboratory using a novel near-infrared sensitive InGaAs 2D-array detector. An imaging spectrograph has been inserted in front of the 2D-array detector, which allows us to acquire spectral images where one dimension is spatial and the other is spectral. The work presents a detailed examination of sensitivity and noise characteristics of the setup and its ability to detect O-1(2). The O-1(2') phosphorescence-based images and near-infrared luminescence spectral images recorded from single TMPyP-containing fibroblast cells reflecting spectral changes during irradiation are demonstrated. The introduction of spectral images addresses the issue of a potential spectral overlap of O-1(2) phosphorescence with near-infrared-extended luminescence of the photosensitizer and provides a powerful tool for distinguishing and separating them, which can be applied to any photosensitizer manifesting near-infrared luminescence." . "5"^^ . . . . . . . "Photochemical and Photobiological Sciences" . "Breitenbach, Thomas" . . . . . "10"^^ . "4"^^ . . . "Scholz, Marek" . . "1474-905X" . "8" . "10.1039/c4pp00121d" . "RIV/00216208:11320/14:10286185" . . . "Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells" . "http://dx.doi.org/10.1039/c4pp00121d" . "Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells" . "000341296500012" . . "Valenta, Jan" . "Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells"@en . "D\u011Bdic, Roman" . . "Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells"@en . . . "RIV/00216208:11320/14:10286185!RIV15-MSM-11320___" . "41497" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "[34FCCD5D9868]" . "A new setup for direct microspectroscopic monitoring of singlet oxygen (O-1(2)) has been developed in our laboratory using a novel near-infrared sensitive InGaAs 2D-array detector. An imaging spectrograph has been inserted in front of the 2D-array detector, which allows us to acquire spectral images where one dimension is spatial and the other is spectral. The work presents a detailed examination of sensitivity and noise characteristics of the setup and its ability to detect O-1(2). The O-1(2') phosphorescence-based images and near-infrared luminescence spectral images recorded from single TMPyP-containing fibroblast cells reflecting spectral changes during irradiation are demonstrated. The introduction of spectral images addresses the issue of a potential spectral overlap of O-1(2) phosphorescence with near-infrared-extended luminescence of the photosensitizer and provides a powerful tool for distinguishing and separating them, which can be applied to any photosensitizer manifesting near-infrared luminescence."@en . "13" . . "11320" . "ccd; lifetime; diffusion; sensitizer; in-vivo; optical-detection; delayed fluorescence; water-soluble photosensitizers"@en . "H\u00E1la, Jan" . .