. . "Praus, Petr" . "Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution" . "\u0160t\u011Bp\u00E1nek, Josef" . . "Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution" . "5"^^ . "11320" . "Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell." . "259806" . . . "24" . . "Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution"@en . . "3-4" . "Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution"@en . "0712-4813" . . . . . "Mojze\u0161, Peter" . "Z(MSM0021620835)" . "NL - Nizozemsko" . "000281667400019" . . . "6"^^ . . "RIV/00216208:11320/10:10070564" . "Turpin, Pierre-Yves" . . . . "Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell."@en . "Ko\u010Di\u0161ov\u00E1, Eva" . "[51206013413F]" . . "4"^^ . "SPECTROSCOPY-AN INTERNATIONAL JOURNAL" . . "RIV/00216208:11320/10:10070564!RIV11-MSM-11320___" . . "Sureau, Franck" . "cellular uptake; microspectrofluorometry; porphyrin; oligonucleotide; Fluorescence lifetime"@en .