"290412" . "Biochemical and Biophysical Research Communications" . "Zentz, Christian" . "2"^^ . . "[BC0409E7E340]" . "Turpin, Pierre-Yves" . . . "000273624500037" . "Structural and dynamic changes of the serum response element and the core domain of serum response factor induced by their association"@en . . . . "Alpert, Bernard" . "Transcriptional activity of serum response factor (SRF) is dependent on its binding to the CC(A/T)6GG box (CArG box) of serum response element (SRE). By Raman spectroscopy, we carried out a comparative analysis, in solution, of the complexes obtained from the association of core-SRF with 20-mer SREs bearing wild-type and mutated c-fos CArG boxes. In case of association with the wild type c-fos CArG box, the complex does not bring out the expected Raman signature of a stable bending of the targeted SRE but keeps a bend-linear conformer oligonucleotide interconversion. The linear conformer population is larger than that of free oligonucleotide. In the core-SRF moiety of the wild-type complex a large spectral change associated with the CO-groups from Asp and/or Glu residues shows that their ionization states and the strength of their interactions decrease as compared to those of mutated non-specific complexes."@en . . "Mezzetti, Alberto" . "Paulin, Denise" . "RIV/00216208:11320/10:10070495" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Raman spectroscopy; specific recognition; c-fos; SRE; core-SRF"@en . "\u0160t\u011Bp\u00E1nek, Josef" . "11320" . . . "6"^^ . . "Kopeck\u00FD, Vladim\u00EDr" . "Structural and dynamic changes of the serum response element and the core domain of serum response factor induced by their association" . . "Structural and dynamic changes of the serum response element and the core domain of serum response factor induced by their association" . . . "7"^^ . "391" . "0006-291X" . "Structural and dynamic changes of the serum response element and the core domain of serum response factor induced by their association"@en . "1" . . . "Transcriptional activity of serum response factor (SRF) is dependent on its binding to the CC(A/T)6GG box (CArG box) of serum response element (SRE). By Raman spectroscopy, we carried out a comparative analysis, in solution, of the complexes obtained from the association of core-SRF with 20-mer SREs bearing wild-type and mutated c-fos CArG boxes. In case of association with the wild type c-fos CArG box, the complex does not bring out the expected Raman signature of a stable bending of the targeted SRE but keeps a bend-linear conformer oligonucleotide interconversion. The linear conformer population is larger than that of free oligonucleotide. In the core-SRF moiety of the wild-type complex a large spectral change associated with the CO-groups from Asp and/or Glu residues shows that their ionization states and the strength of their interactions decrease as compared to those of mutated non-specific complexes." . "RIV/00216208:11320/10:10070495!RIV11-MSM-11320___" . . "Z(MSM0021620835)" . . .