"Stanoven\u00ED kinetiky Michaelis-Mentenov\u00E9 pro definovan\u00E9 substr\u00E1ty v experiment\u00E1ln\u00EDch syst\u00E9mech. Stanoven\u00ED vazebn\u00FDch parametr\u016F v supersom\u00E1ln\u00ED frakci jedn\u00E9 lidsk\u00E9 formy cytochromu P450 pro aktivn\u00ED farmaceutickou substanci"@cs . "[80B6F02C1B9A]" . "RIV/00216208:11310/14:10292510" . "The contractual research included experimental in vitro characterization of enzymatic kinetics and binding affinity test for two pharmaceutically active substances and two medicinal drug forms in three relevant enzymatic systems. The three enzymatic preparations, two human microsomal fractions (male, female) and one isoform of human cytochrome P450 were employed for experimental approaches. With tested substances was optimized experimental work-flow including incubations with enzymatic substrates, extraction protocol, analytical separation employing HPLC with UV-VIS or MS detection. The obtained values were used for quantitative and qualitative evaluation and determination of kinetics parameters."@en . "1"^^ . "Stanoven\u00ED kinetiky Michaelis-Mentenov\u00E9 pro definovan\u00E9 substr\u00E1ty v experiment\u00E1ln\u00EDch syst\u00E9mech. Stanoven\u00ED vazebn\u00FDch parametr\u016F v supersom\u00E1ln\u00ED frakci jedn\u00E9 lidsk\u00E9 formy cytochromu P450 pro aktivn\u00ED farmaceutickou substanci" . . . . . "Stanoven\u00ED kinetiky Michaelis-Mentenov\u00E9 pro definovan\u00E9 substr\u00E1ty v experiment\u00E1ln\u00EDch syst\u00E9mech. Stanoven\u00ED vazebn\u00FDch parametr\u016F v supersom\u00E1ln\u00ED frakci jedn\u00E9 lidsk\u00E9 formy cytochromu P450 pro aktivn\u00ED farmaceutickou substanci"@cs . . "Stanoven\u00ED kinetiky Michaelis-Mentenov\u00E9 pro definovan\u00E9 substr\u00E1ty v experiment\u00E1ln\u00EDch syst\u00E9mech. Stanoven\u00ED vazebn\u00FDch parametr\u016F v supersom\u00E1ln\u00ED frakci jedn\u00E9 lidsk\u00E9 formy cytochromu P450 pro aktivn\u00ED farmaceutickou substanci" . . "cytochrome P450; binding affinity test; enzymatic kinetic"@en . . . "Smluvn\u00ED v\u00FDzkum zahrnoval experiment\u00E1ln\u00ED in vitro charakterizaci enzymov\u00E9 kinetiky a vazebn\u00E9 studie pro dv\u011B farmaceuticky aktivn\u00ED substance a dv\u011B l\u00E9kov\u00E9 formy ve t\u0159ech biochemicky relevantn\u00EDch enzymov\u00FDch syst\u00E9mech. Pro experiment\u00E1ln\u00ED p\u0159\u00EDstupy byly pou\u017Eity t\u0159i enzymov\u00E9 prepar\u00E1ty, lidsk\u00E1 mikrosom\u00E1ln\u00ED frakce (\u017Eena, mu\u017E) a supersom\u00E1ln\u00ED frakce jedn\u00E9 lidsk\u00E9 formy cytochromu P450. S testovan\u00FDmi substancemi byly provedeny po p\u0159edchoz\u00ED optimalizaci inkuba\u010Dn\u00ED experimenty s enzymov\u00FDmi prepar\u00E1ty, extrakce, analytick\u00E9 stanoven\u00ED tvo\u0159en\u00FDch produkt pomoc\u00ED HPLC s detekc\u00ED UV-VIS \u010Di MS. Kvantitativn\u00ED a kvalitativn\u00ED vyhodnocen\u00ED a ur\u010Den\u00ED kinetick\u00FDch parametr\u016F ze z\u00EDskan\u00FDch hodnot."@cs . "Determination of Michaelis-Menten characteristics for defined substrates in experimental systems. Determination of binding characteristics of pharmaceutically active form in supersomal fraction of one human cytochrome P-450 form"@en . "Smluvn\u00ED v\u00FDzkum zahrnoval experiment\u00E1ln\u00ED in vitro charakterizaci enzymov\u00E9 kinetiky a vazebn\u00E9 studie pro dv\u011B farmaceuticky aktivn\u00ED substance a dv\u011B l\u00E9kov\u00E9 formy ve t\u0159ech biochemicky relevantn\u00EDch enzymov\u00FDch syst\u00E9mech. Pro experiment\u00E1ln\u00ED p\u0159\u00EDstupy byly pou\u017Eity t\u0159i enzymov\u00E9 prepar\u00E1ty, lidsk\u00E1 mikrosom\u00E1ln\u00ED frakce (\u017Eena, mu\u017E) a supersom\u00E1ln\u00ED frakce jedn\u00E9 lidsk\u00E9 formy cytochromu P450. S testovan\u00FDmi substancemi byly provedeny po p\u0159edchoz\u00ED optimalizaci inkuba\u010Dn\u00ED experimenty s enzymov\u00FDmi prepar\u00E1ty, extrakce, analytick\u00E9 stanoven\u00ED tvo\u0159en\u00FDch produkt pomoc\u00ED HPLC s detekc\u00ED UV-VIS \u010Di MS. Kvantitativn\u00ED a kvalitativn\u00ED vyhodnocen\u00ED a ur\u010Den\u00ED kinetick\u00FDch parametr\u016F ze z\u00EDskan\u00FDch hodnot." . "\u0160ulc, Miroslav" . "Neuveden" . "11310" . . "N" . "RIV/00216208:11310/14:10292510!RIV15-MSM-11310___" . "9"^^ . "Determination of Michaelis-Menten characteristics for defined substrates in experimental systems. Determination of binding characteristics of pharmaceutically active form in supersomal fraction of one human cytochrome P-450 form"@en . . . . "Zentiva, k.s., Praha 10, Doln\u00ED M\u011Bcholupy, U kabelovny 130, PS\u010C 102 37" . . "1"^^ . . "47115" .