. "[9A81BDE82026]" . . "10.1007/s11738-014-1573-1" . . "11310" . "36" . . "8" . . . "We describe complex multiple concentration dependencies for the response of isolated pith tissues to plant biologically active substances. Kale and tobacco stem pith explants were cultured on agar media containing combinations of sucrose, cytokinin [kinetin or benzyladenine (BA)] and auxin [indole-3-acetic acid (IAA) or naphthaleneacetic acid (NAA)]. Absorption of these components by explants and their effects on explant mass, contents of soluble proteins, starch and sugars, and activity of ADP-glucose pyrophosphorylase (AGPase) were studied in relation to their concentration. Up to ten pronounced statistically significant maxima (peaks or waves) were repeatedly detected in the dose-response curves over a concentration range of several logarithmic orders. Slight maxima were observed in the corresponding absorption curves. Pronounced maxima of sucrose absorption were induced by IAA and BA, and those of NAA absorption were induced by sucrose. Both types of multiple maxima (in dose-response and absorption curves) may be due to changes in concentration of intracellular solutes (sugars, auxins and cytokinins), thereby affecting metabolic processes that act as sinks for external solutes and elicit feedback appearance of maxima in absorption curves. Good correspondence between external concentrations at which maxima of different compared curves occur in addition to statistical significance of individual maxima and repeatability of experimental results supports the conclusion that the multiple maxima exhibited are genuine. We consider it possibile that the multiple maxima are associated with endopolyploidy or mixoploidy and/or epigenomic diversity of pith cells that show different sensitivities to biologically active solutes." . . "\u017D\u00E1rsk\u00FD, Viktor" . . . . . "Acta Physiologiae Plantarum" . "http://dx.doi.org/10.1007/s11738-014-1573-1" . "0137-5881" . "2"^^ . . "Cizkova, Jana" . . "Kaminek, Miroslav" . "Multiple, concentration-dependent effects of sucrose, auxins and cytokinins in explant cultures of kale and tobacco" . . "Multiple, concentration-dependent effects of sucrose, auxins and cytokinins in explant cultures of kale and tobacco" . "RIV/00216208:11310/14:10281960!RIV15-MSM-11310___" . "Multiple, concentration-dependent effects of sucrose, auxins and cytokinins in explant cultures of kale and tobacco"@en . "31081" . . "I, P(LO1204)" . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Multiple, concentration-dependent effects of sucrose, auxins and cytokinins in explant cultures of kale and tobacco"@en . . "11"^^ . . "Dolezel, Jaroslav" . "Sugars; Starch accumulation; Soluble proteins; Mixoploidy; Epigenomic diversity; Endopolyploidy; AGPase; Absorption; Nicotiana tabacum; Brassica oleracea"@en . "6"^^ . . "000339817600003" . "Cvr\u010Dkov\u00E1, Fatima" . "RIV/00216208:11310/14:10281960" . . . . "We describe complex multiple concentration dependencies for the response of isolated pith tissues to plant biologically active substances. Kale and tobacco stem pith explants were cultured on agar media containing combinations of sucrose, cytokinin [kinetin or benzyladenine (BA)] and auxin [indole-3-acetic acid (IAA) or naphthaleneacetic acid (NAA)]. Absorption of these components by explants and their effects on explant mass, contents of soluble proteins, starch and sugars, and activity of ADP-glucose pyrophosphorylase (AGPase) were studied in relation to their concentration. Up to ten pronounced statistically significant maxima (peaks or waves) were repeatedly detected in the dose-response curves over a concentration range of several logarithmic orders. Slight maxima were observed in the corresponding absorption curves. Pronounced maxima of sucrose absorption were induced by IAA and BA, and those of NAA absorption were induced by sucrose. Both types of multiple maxima (in dose-response and absorption curves) may be due to changes in concentration of intracellular solutes (sugars, auxins and cytokinins), thereby affecting metabolic processes that act as sinks for external solutes and elicit feedback appearance of maxima in absorption curves. Good correspondence between external concentrations at which maxima of different compared curves occur in addition to statistical significance of individual maxima and repeatability of experimental results supports the conclusion that the multiple maxima exhibited are genuine. We consider it possibile that the multiple maxima are associated with endopolyploidy or mixoploidy and/or epigenomic diversity of pith cells that show different sensitivities to biologically active solutes."@en . . "Lustinec, Ji\u0159\u00ED" . .