. "Javorkov\u00E1, Eli\u0161ka" . "Distinct cytokines balance the development of regulatory T cells and interleukin-10-producing regulatory B cells"@en . "http://dx.doi.org/10.1111/imm.12219" . "[FAEC1D559D21]" . "immunosuppression; interleukin-10 production; cytokines; B cells; autoregulation"@en . "RIV/00216208:11310/14:10272121" . "Zajicov\u00E1, Alena" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "10.1111/imm.12219" . "13"^^ . . "Hol\u00E1\u0148, Vladim\u00EDr" . "Pavl\u00EDkov\u00E1, Michaela" . . . . "Distinct cytokines balance the development of regulatory T cells and interleukin-10-producing regulatory B cells" . . . . . . "141" . . "000332474100010" . . . "Krulov\u00E1, Magdal\u00E9na" . . "Regulatory T cells have been well described and the factors regulating their development and function have been identified. Recently, a growing body of evidence has documented the existence of interleukin-10 (IL-10) -producing B cells, which are called regulatory B10 cells. These cells attenuate autoimmune, inflammatory and transplantation reactions, and the main mechanism of their inhibitory action is the production of IL-10. We show that the production of IL-10 by lipopolysaccharide-stimulated B cells is significantly enhanced by IL-12 and interferon- and negatively regulated by IL-21 and transforming growth factor-. In addition, exogenous IL-10 also inhibits B-cell proliferation and the expression of the IL-10 gene in lipopolysaccharide-stimulated B cells. The negative autoregulation of IL-10 production is supported by the observation that the inclusion of anti-IL-10 receptor monoclonal antibody enhances IL-10 production and the proliferation of activated B cells. The effects of cytokines on IL-10 production by B10 cells did not correlate with their effects on B-cell proliferation or on IL-10 production by T cells or macrophages. The cytokine-induced changes in IL-10 production occurred on the level of IL-10 gene expression, as confirmed by increased or decreased IL-10 mRNA expression in the presence of a particular cytokine. The regulatory cytokines modulate the number of IL-10-producing cells rather than augmenting or decreasing the secretion of IL-10 on a single-cell level. Altogether these data show that the production of IL-10 by B cells is under the strict regulatory control of cytokines and that individual cytokines differentially regulate the development and activity of regulatory T cells and IL-10-producing regulatory B cells."@en . . . . "4" . "Regulatory T cells have been well described and the factors regulating their development and function have been identified. Recently, a growing body of evidence has documented the existence of interleukin-10 (IL-10) -producing B cells, which are called regulatory B10 cells. These cells attenuate autoimmune, inflammatory and transplantation reactions, and the main mechanism of their inhibitory action is the production of IL-10. We show that the production of IL-10 by lipopolysaccharide-stimulated B cells is significantly enhanced by IL-12 and interferon- and negatively regulated by IL-21 and transforming growth factor-. In addition, exogenous IL-10 also inhibits B-cell proliferation and the expression of the IL-10 gene in lipopolysaccharide-stimulated B cells. The negative autoregulation of IL-10 production is supported by the observation that the inclusion of anti-IL-10 receptor monoclonal antibody enhances IL-10 production and the proliferation of activated B cells. The effects of cytokines on IL-10 production by B10 cells did not correlate with their effects on B-cell proliferation or on IL-10 production by T cells or macrophages. The cytokine-induced changes in IL-10 production occurred on the level of IL-10 gene expression, as confirmed by increased or decreased IL-10 mRNA expression in the presence of a particular cytokine. The regulatory cytokines modulate the number of IL-10-producing cells rather than augmenting or decreasing the secretion of IL-10 on a single-cell level. Altogether these data show that the production of IL-10 by B cells is under the strict regulatory control of cytokines and that individual cytokines differentially regulate the development and activity of regulatory T cells and IL-10-producing regulatory B cells." . "11310" . "7"^^ . "6"^^ . . "11800" . "I, P(GAP301/11/1568), P(GAP304/11/0653), S, Z(MSM0021620858)" . . . . "RIV/00216208:11310/14:10272121!RIV15-MSM-11310___" . "Immunology" . . "Distinct cytokines balance the development of regulatory T cells and interleukin-10-producing regulatory B cells"@en . . . . . "Distinct cytokines balance the development of regulatory T cells and interleukin-10-producing regulatory B cells" . "0019-2805" . . "Chud\u00ED\u010Dkov\u00E1, Milada" . "Tro\u0161an, Peter" .