. "Ku\u010Derov\u00E1, Zdenka" . "I, P(GA203/09/0857), P(LC06044), Z(AV0Z40500505), Z(MSM0021620806)" . "RIV/00216208:11310/12:10126758!RIV13-MSM-11310___" . . . . "5"^^ . . . . "Lenfeld, Ji\u0159\u00ED" . "P\u0159ikryl, Petr" . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "11310" . "Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin" . . . . "Hor\u00E1k, Daniel" . . . "1"^^ . "0273-2289" . "10.1007/s12010-012-9772-y" . "Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin"@en . . . . "RIV/00216208:11310/12:10126758" . . . . . . "http://dx.doi.org/10.1007/s12010-012-9772-y" . . "2" . "000309224300007" . . "Tich\u00E1, Marie" . "[A1B1748358E6]" . . "Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol-gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of alpha-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. alpha-Chymotrypsin immobilized to magnetic bead cellulose was used for the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown." . "Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin" . . "147748" . "11"^^ . "proteomics; microspheres; digestion; trypsin; porcine pepsin A; magnetic bead cellulose; immobilized alpha-chymotrypsin; human gastric juice; enzyme immobilization"@en . "Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology" . . "168" . "Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol-gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of alpha-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. alpha-Chymotrypsin immobilized to magnetic bead cellulose was used for the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown."@en . "Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin"@en .