. "Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode" . . "Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode"@en . "3" . . . "130668" . "1618-2642" . . . . . . "3"^^ . "http://dx.doi.org/10.1007/s00216-012-6140-2" . "Analytical and Bioanalytical Chemistry" . "[39F066EC3AA8]" . "10.1007/s00216-012-6140-2" . "RIV/00216208:11310/12:10126406" . "Vysko\u010Dil, Vlastimil" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Human urine; Solid-phase extraction; Boron-doped diamond film electrode; High-performance liquid chromatography with electrochemical detection; 1-Hydroxypyrene"@en . . . . "3"^^ . "I, P(GBP206/12/G151), P(ME10004), S, Z(MSM0021620857)" . "000307251900011" . "RIV/00216208:11310/12:10126406!RIV13-GA0-11310___" . . . . "7"^^ . "Barek, Ji\u0159\u00ED" . "Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode" . . "404" . . "11310" . . "Yosypchuk, Oksana" . . "A high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) at a boron-doped diamond film electrode with preliminary separation and preconcentration by solid-phase extraction (SPE) has been developed for the determination of 1-hydroxypyrene (1-HP) in human urine. 1-HP is among the most widely used biomarkers of exposure to polycyclic aromatic hydrocarbons. Optimal HPLC-ED conditions have been found: mobile phase methanol-0.05 mol L-1 phosphate buffer pH 5.0 (80:20, v/v), detection potential +1,000 mV versus Ag/AgCl (3 mol L-1 KCl), and flow rate 0.8 mL min(-1). For SPE, LiChrolut(A (R)) RP-18 E cartridges were used. The extraction yield was (87.0 A +/- 5.8) % (n = 5). The concentration dependence of 1-HP was measured in the concentration range from 0.01 to 10 mu mol L-1 (2.18-2,180 mu g L-1) using methanolic solutions resulting from the SPE pretreatment of spiked human urine samples. The limit of detection (signal-to-noise ratio 3) and the limit of quantification (signal-to-noise ratio 10) of the biomarker were 0.013 mu mol L-1 (2.84 mu g L-1) and 0.043 mu mol L-1 (9.39 mu g L-1), respectively, which is sufficient for its determination in the urine of persons exposed to polycyclic aromatic hydrocarbons."@en . "A high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) at a boron-doped diamond film electrode with preliminary separation and preconcentration by solid-phase extraction (SPE) has been developed for the determination of 1-hydroxypyrene (1-HP) in human urine. 1-HP is among the most widely used biomarkers of exposure to polycyclic aromatic hydrocarbons. Optimal HPLC-ED conditions have been found: mobile phase methanol-0.05 mol L-1 phosphate buffer pH 5.0 (80:20, v/v), detection potential +1,000 mV versus Ag/AgCl (3 mol L-1 KCl), and flow rate 0.8 mL min(-1). For SPE, LiChrolut(A (R)) RP-18 E cartridges were used. The extraction yield was (87.0 A +/- 5.8) % (n = 5). The concentration dependence of 1-HP was measured in the concentration range from 0.01 to 10 mu mol L-1 (2.18-2,180 mu g L-1) using methanolic solutions resulting from the SPE pretreatment of spiked human urine samples. The limit of detection (signal-to-noise ratio 3) and the limit of quantification (signal-to-noise ratio 10) of the biomarker were 0.013 mu mol L-1 (2.84 mu g L-1) and 0.043 mu mol L-1 (9.39 mu g L-1), respectively, which is sufficient for its determination in the urine of persons exposed to polycyclic aromatic hydrocarbons." . "Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode"@en . . . . . . .