"[958ABBD7B8ED]" . . . . . "Development and differentiation of Th cells is highly plastic and strictly regulated by cytokines. We show negative regulation of TGF-beta-dependent differentiation programs by IL-12. Development of TGF-b-induced regulatory T cells (iTregs) or TGF-b/IL-6-activated Th17 cells from purified mouse CD4+CD25- T cells stimulated with anti-CD3 was abrogated by IL-12, establishing different developmental program. IL-12 inhibited expression of lineage-specific transcription factors Foxp3 and RORgt in developing Tregs and Th17 cells. IL-12 altered development of iTregs and Th17 cells even added after 48 h. Cells activated by TGF-b and IL-12 had increased expression of T-bet, produced Th1 cytokines IFN-g and IL-2 and expressed IL-18 receptor and C-C chemokine receptor type 5. Cells activated by both TGF-b and IL-12 stimulated macrophages to produce nitric oxide. IL-12 is shown as superior cytokine able to skew ongoing TGF-b-dependent iTreg or Th17 developmental program to Th1-like direction."@en . . "217" . . . "9"^^ . . "Hol\u00E1\u0148, Vladim\u00EDr" . "Development and differentiation of Th cells is highly plastic and strictly regulated by cytokines. We show negative regulation of TGF-beta-dependent differentiation programs by IL-12. Development of TGF-b-induced regulatory T cells (iTregs) or TGF-b/IL-6-activated Th17 cells from purified mouse CD4+CD25- T cells stimulated with anti-CD3 was abrogated by IL-12, establishing different developmental program. IL-12 inhibited expression of lineage-specific transcription factors Foxp3 and RORgt in developing Tregs and Th17 cells. IL-12 altered development of iTregs and Th17 cells even added after 48 h. Cells activated by TGF-b and IL-12 had increased expression of T-bet, produced Th1 cytokines IFN-g and IL-2 and expressed IL-18 receptor and C-C chemokine receptor type 5. Cells activated by both TGF-b and IL-12 stimulated macrophages to produce nitric oxide. IL-12 is shown as superior cytokine able to skew ongoing TGF-b-dependent iTreg or Th17 developmental program to Th1-like direction." . . "P(1M0506), P(GAP301/11/1568), P(GAP304/11/0653), P(GD310/08/H077), P(KAN200520804), S, Z(AV0Z50520514), Z(MSM0021620858)" . . . . "T cell subsets; T cell differentiation; cytokines"@en . . "1" . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . . "3"^^ . "RIV/00216208:11310/12:10104201" . . "http://dx.doi.org/10.1016/j.imbio.2011.07.032" . . . "140479" . "RIV/00216208:11310/12:10104201!RIV13-MSM-11310___" . . "3"^^ . . "Pokorn\u00E1, Kate\u0159ina" . "0171-2985" . . "IL-12 inhibits the TGF-beta-dependent T cell developmental programs and skews the TGF-beta-induced differentiation into a Th1-like direction"@en . "Immunobiology" . . "IL-12 inhibits the TGF-beta-dependent T cell developmental programs and skews the TGF-beta-induced differentiation into a Th1-like direction"@en . . "Proch\u00E1zkov\u00E1, Jana" . "IL-12 inhibits the TGF-beta-dependent T cell developmental programs and skews the TGF-beta-induced differentiation into a Th1-like direction" . . "IL-12 inhibits the TGF-beta-dependent T cell developmental programs and skews the TGF-beta-induced differentiation into a Th1-like direction" . . "10.1016/j.imbio.2011.07.032" . "000299445400011" . "11310" .