. . "Fischer, Luk\u00E1\u0161" . . "Annals of Botany" . "0305-7364" . . "Opatrn\u00FD, Zden\u011Bk" . "8"^^ . . "4" . . . . "3"^^ . "106" . "RIV/00216208:11310/10:10082159!RIV11-MSM-11310___" . . "000282167400006" . "11310" . "Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation"@en . "Nocarov\u00E1, Eva" . "[5D513A2DA03B]" . "3"^^ . "P(LC06004), P(LC06034), Z(MSM0021620858)" . . "GFP; potato (Solanum tuberosum); transgene silencing"@en . "Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation"@en . "Transgenic plants represent an excellent tool for experimental plant biology and are an important component of modern agriculture. Transgenes can be silenced long after their integration. To study the long-term changes in transgene expression in potato, the activity of two reporter genes, encoding GFP and NPTII, was monitored in a set of 17 transgenic lines over 5 years of vegetative propagation in vitro. Complete silencing of the reporter genes was observed in four lines (nearly 25 %), all of which successively silenced the two reporter genes, indicating an interconnection between their silencing. We suggest a hypothetical mechanism involving the successive silencing of the two reporter genes that involves the switch of GFP silencing from the post-transcriptional to transcriptional level and subsequent spreading of methylation to the NPTII gene." . . . . . . "290997" . . . "Transgenic plants represent an excellent tool for experimental plant biology and are an important component of modern agriculture. Transgenes can be silenced long after their integration. To study the long-term changes in transgene expression in potato, the activity of two reporter genes, encoding GFP and NPTII, was monitored in a set of 17 transgenic lines over 5 years of vegetative propagation in vitro. Complete silencing of the reporter genes was observed in four lines (nearly 25 %), all of which successively silenced the two reporter genes, indicating an interconnection between their silencing. We suggest a hypothetical mechanism involving the successive silencing of the two reporter genes that involves the switch of GFP silencing from the post-transcriptional to transcriptional level and subsequent spreading of methylation to the NPTII gene."@en . "Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation" . . . "Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation" . "RIV/00216208:11310/10:10082159" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . .