"A determination of submicromolar concentrations of glycine in periaquecuctal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection" . . "P(GA203/07/0896), Z(MSM0021620814), Z(MSM0021620816)" . "A determination of submicromolar concentrations of glycine in periaquecuctal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection"@en . "RIV/00216208:11310/09:10001398!RIV10-GA0-11310___" . "11310" . "6"^^ . "A determination of submicromolar concentrations of glycine in periaquecuctal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection" . "Samcov\u00E1, Eva" . "Soukupov\u00E1, Marie" . . . . . "Capillary electrophoresis (CE) with contactless conductivity detection (C4D) has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The limit of detection for glycine has been decreased to a value of 0.2 ?M by adding 75% v/v of acetonitrile to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/C4D method makes it possible to determine micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared to a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol."@en . "Capillary electrophoresis (CE) with contactless conductivity detection (C4D) has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The limit of detection for glycine has been decreased to a value of 0.2 ?M by adding 75% v/v of acetonitrile to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/C4D method makes it possible to determine micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared to a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol." . "A determination of submicromolar concentrations of glycine in periaquecuctal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection"@en . "\u0160tul\u00EDk, Karel" . . . "[58B228D3AADA]" . . . "30" . "301239" . . "19" . . "0173-0835" . . . "1"^^ . . "Electrophoresis" . . "Amino acids; Contactless conductivity detection; Neurotransmitters"@en . "RIV/00216208:11310/09:10001398" . . "4"^^ . "T\u016Fma, Petr" . . . "DE - Spolkov\u00E1 republika N\u011Bmecko" .