. "Cytoplasmic helicase encoded by pGKL plasmids" . "Yeast" . . "3"^^ . "Posp\u00ED\u0161ek, Martin" . "11310" . "3"^^ . "0"^^ . . "Cytoplasmic helicase encoded by pGKL plasmids"@en . "0"^^ . "Pejznochov\u00E1, Martina" . "For production of putative helicase we used T7-based system and despite of difficulty with production of AT rich sequences we successfully expressed ORF4p helicase in E. coli. Further, we partly purified ORF4p helicase by affinity chromatography" . "Cytoplasmic helicase encoded by pGKL plasmids" . "RIV/00216208:11310/03:00001306!RIV/2004/GA0/113104/N" . . . . . "0749-503X" . . . "602582" . . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "RIV/00216208:11310/03:00001306" . "P(GA204/03/1487), Z(MSM 113100003)" . . . "20" . . "SI" . . "For production of putative helicase we used T7-based system and despite of difficulty with production of AT rich sequences we successfully expressed ORF4p helicase in E. coli. Further, we partly purified ORF4p helicase by affinity chromatography"@en . "1"^^ . . "[DBD861FCE14E]" . "325;325" . "Cytoplasmic;helicase;encoded;plasmids"@en . "Cytoplasmic helicase encoded by pGKL plasmids"@en . "Vop\u00E1lensk\u00FD, V\u00E1clav" . . . .