"34601" . "On-line SPE-UHPLC method using fused core columns for extraction and separation of nine illegal dyes in chilli-containing spices" . "4"^^ . "9"^^ . "000341897800061" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "On-line SPE-UHPLC method using fused core columns for extraction and separation of nine illegal dyes in chilli-containing spices"@en . . "\u0160at\u00EDnsk\u00FD, Dalibor" . "Talanta" . "RIV/00216208:11160/14:10282252" . "The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes Sudan I-IV, Sudan Red 7B, Sudan Red G, Sudan Orange G, Para Red, and Methyl Red were separated and analyzed in less than 9 min without labor-consuming pretreatment procedure. The chromatographic separation was performed on Ascentis Express RP-Amide column with gradient elution using mixture of acetonitrile and water, as a mobile phase at a flow rate of 1.0 mL min(-1) and 55 degrees C of temperature. As SPE sorbent for cleanup and pre-concentration of illegal dyes short guard fused core column Ascentis Express F5 was used. The applicability of proposed method was proven for three different chilli-containing commercial samples. Recoveries for all compounds were between 90% and 108% and relative standard deviation ranged from 1% to 4% for within- and from 2% to 6% for between-day. Limits of detection showed lower values than required by European Union regulations and were in the range of 3.3-10.3 mu g L-1 for standard solutions, 5.6-235.6 mu g kg(-1) for chilli-containing spices." . "http://www.sciencedirect.com/science/article/pii/S0039914014005943" . . "Chilli-containing samples; Fused core column; Illegal red Sudan dyes; UHPLC; Column switching; On-line solid phase extraction"@en . "0039-9140" . . . "The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes Sudan I-IV, Sudan Red 7B, Sudan Red G, Sudan Orange G, Para Red, and Methyl Red were separated and analyzed in less than 9 min without labor-consuming pretreatment procedure. The chromatographic separation was performed on Ascentis Express RP-Amide column with gradient elution using mixture of acetonitrile and water, as a mobile phase at a flow rate of 1.0 mL min(-1) and 55 degrees C of temperature. As SPE sorbent for cleanup and pre-concentration of illegal dyes short guard fused core column Ascentis Express F5 was used. The applicability of proposed method was proven for three different chilli-containing commercial samples. Recoveries for all compounds were between 90% and 108% and relative standard deviation ranged from 1% to 4% for within- and from 2% to 6% for between-day. Limits of detection showed lower values than required by European Union regulations and were in the range of 3.3-10.3 mu g L-1 for standard solutions, 5.6-235.6 mu g kg(-1) for chilli-containing spices."@en . . . "Khalikova, Maria A." . . . . . . "\u0160midrkalov\u00E1, Tereza" . . . "Solich, Petr" . "On-line SPE-UHPLC method using fused core columns for extraction and separation of nine illegal dyes in chilli-containing spices"@en . "I, P(EE2.3.20.0235)" . . "RIV/00216208:11160/14:10282252!RIV15-MSM-11160___" . . . . . "130" . . "December" . "11160" . "[C6FD65C44E06]" . "4"^^ . "10.1016/j.talanta.2014.07.038" . . "On-line SPE-UHPLC method using fused core columns for extraction and separation of nine illegal dyes in chilli-containing spices" .