"3"^^ . "107" . . . . "11150" . "0009-2770" . . . . . "Multiplex Dideoxynucleotide Sequencing Using Polyadenylated Sequencing Primers Multiplex Dideoxynucleotide Sequencing Using Polyadenylated Sequencing Primers"@en . . "Multiplex Dideoxynucleotide Sequencing Using Polyadenylated Sequencing Primers Multiplex Dideoxynucleotide Sequencing Using Polyadenylated Sequencing Primers"@en . . "1" . "Multiplexn\u00ED dideoxynukleotidov\u00E9 sekvenov\u00E1n\u00ED s polyadenylovan\u00FDmi sekvena\u010Dn\u00EDmi primery" . "CZ - \u010Cesk\u00E1 republika" . "RIV/00216208:11150/13:10174039!RIV14-MSM-11150___" . "4"^^ . "http://www.chemicke-listy.cz/docs/full/2013_01_62-65.pdf" . "Chemick\u00E9 listy" . "The new approach is used for identification of C282Y (C), H63D (H), and S65C (S) mutations in the HFE gene. In a group of 40 samples we amplified specific parts of HFE exon 2 and exon 4 by multiplex PCR. Purified amplicons were subsequently used in a multiplex cycle sequencing reaction containing a hyperadenylated reverse primer for exon 2. Adenylation prolonged the migration time of the exon 2 amplicons in the electrophoretic capillary. This enabled us to obtain separated sequencing data for both the studied amplicons. We found six subjects with the risk of hereditary hemochromatosis (15 %)."@en . "V na\u0161\u00ED pr\u00E1ci jsme pou\u017Eili nov\u00FD postup pro identifikaci mutac\u00ED C282Y, H63D a S65C v genu HFE. Ve skupin\u011B 40 vzork\u016F jsme amplifikovali \u010D\u00E1sti HFE genu v exonech 2 a 4 multiplexn\u00ED PCR. Purifikovan\u00E9 amplikony byly n\u00E1sledn\u011B pou\u017Eity do multiplexn\u00ED sekvena\u010Dn\u00ED reakce s hyperadenylovan\u00FDm reverzn\u00EDm primerem pro exon 2. Adenylace prodlou\u017Eila migra\u010Dn\u00ED \u010Das produktu exonu 2 p\u0159i kapil\u00E1rn\u00ED elektrofor\u00E9ze. To umo\u017Enilo z\u00EDskat separovan\u00E1 sekvena\u010Dn\u00ED data pro oba studovan\u00E9 amplikony. Nalezli jsme 6 osob s rizikem heredit\u00E1rn\u00ED hemochromatosy (15 %)."@cs . "Petera, Ji\u0159\u00ED" . "adenylation; sequencing; HFE"@en . . . "Multiplexn\u00ED dideoxynukleotidov\u00E9 sekvenov\u00E1n\u00ED s polyadenylovan\u00FDmi sekvena\u010Dn\u00EDmi primery"@cs . . . "V na\u0161\u00ED pr\u00E1ci jsme pou\u017Eili nov\u00FD postup pro identifikaci mutac\u00ED C282Y, H63D a S65C v genu HFE. Ve skupin\u011B 40 vzork\u016F jsme amplifikovali \u010D\u00E1sti HFE genu v exonech 2 a 4 multiplexn\u00ED PCR. Purifikovan\u00E9 amplikony byly n\u00E1sledn\u011B pou\u017Eity do multiplexn\u00ED sekvena\u010Dn\u00ED reakce s hyperadenylovan\u00FDm reverzn\u00EDm primerem pro exon 2. Adenylace prodlou\u017Eila migra\u010Dn\u00ED \u010Das produktu exonu 2 p\u0159i kapil\u00E1rn\u00ED elektrofor\u00E9ze. To umo\u017Enilo z\u00EDskat separovan\u00E1 sekvena\u010Dn\u00ED data pro oba studovan\u00E9 amplikony. Nalezli jsme 6 osob s rizikem heredit\u00E1rn\u00ED hemochromatosy (15 %)." . "90155" . "Ber\u00E1nek, Martin" . "000314263100011" . . . "Multiplexn\u00ED dideoxynukleotidov\u00E9 sekvenov\u00E1n\u00ED s polyadenylovan\u00FDmi sekvena\u010Dn\u00EDmi primery" . "Drast\u00EDkov\u00E1, Monika" . "Multiplexn\u00ED dideoxynukleotidov\u00E9 sekvenov\u00E1n\u00ED s polyadenylovan\u00FDmi sekvena\u010Dn\u00EDmi primery"@cs . . . "I, P(NT11334)" . "RIV/00216208:11150/13:10174039" . "[64F2A9A84CC9]" . . "3"^^ . .