. . "\u0158ez\u00E1\u010Dov\u00E1, Martina" . "000330226200007" . . . . . "10"^^ . "GR - \u0158eck\u00E1 republika" . "The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction"@en . . . . "Bezrouk, Ale\u0161" . . "Tom\u0161\u00EDk, Pavel" . . . "The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction"@en . "\u0106mielov\u00E1, Jana" . . . . "10"^^ . "67674" . . "In recent years, \u03B1-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of \u03B1-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 \u00B5M but then a recovery of cells occurred. The recovery was not caused by the biotransformation of \u03B1-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of \u03B1-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of \u03B1-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in \u03B1-tomatine-treated cells. These results support the conclusion that \u03B1-tomatine does not induce apoptosis in the MCF-7 cell line." . "10"^^ . . . . "I, P(EE2.3.30.0022), P(GPP303/12/P536), S" . . "Rudolf, Emil" . "[53479F992835]" . "cholesterol; cell death; MCF-7; alpha-tomatine"@en . "RIV/00216208:11150/13:10173671!RIV14-GA0-11150___" . . "In recent years, \u03B1-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of \u03B1-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 \u00B5M but then a recovery of cells occurred. The recovery was not caused by the biotransformation of \u03B1-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of \u03B1-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of \u03B1-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in \u03B1-tomatine-treated cells. These results support the conclusion that \u03B1-tomatine does not induce apoptosis in the MCF-7 cell line."@en . "K\u00F6hlerov\u00E1, Renata" . "11150" . . . . "The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction" . "Hroch, Milo\u0161" . "Oncology Reports" . "10.3892/or.2013.2778" . "Such\u00E1, Lenka" . "http://www.spandidos-publications.com/or/30/6/2593" . "6" . . "RIV/00216208:11150/13:10173671" . "1021-335X" . "\u0160i\u0161pera, Lud\u011Bk" . . "30" . "The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction" . . . . "Havelek, Radim" .