. . . "000314809600005" . . "Tom\u0161\u00EDk, Pavel" . . "Such\u00E1, Lenka" . . "60150" . . . "Journal of Applied Biomedicine" . . "Havelek, Radim" . . "6"^^ . . . "Alpha-tomatine is a major glycoalkaloid found in the roots, leaves, stems and fruit of tomatoes Lycopersicon esculentum. Recently, alpha-tomatine has been recognized as a potential anticancer drug. In the present study, we identified the signaling cascades involved in the antitumor effect of alpha-tomatine on MOLT-4 leukemic cells. Alpha-tomatine inhibited the proliferation and decreased the viability of MOLT-4 cells in a dose-dependent manner. An increase in the activity of caspases 9 and 3/7 was not observed. However, an increase in the amount of p53 and its phosphorylation on serine 15, as well as an increased amount of mitochondrial protein PUMA was detected 4 and 24 h after exposure to alpha-tomatine at a concentration of 1-3 \u03BCmol/l. Inhibition of the proliferation of MOLT-4 cells by alpha-tomatine is also associated with an increase in p21WAF1/CIP1 and the activation of Chk2. The comet assay did not detect significant amounts of single or double DNA strand breaks in cells treated with alpha-tomatine at concentrations of 0.1-9 \u03BCmol/l. Our results thus contribute to the understanding of the anticancer action of alpha-tomatine."@en . "6"^^ . "Seifrtov\u00E1, Martina" . "11150" . "\u0158ez\u00E1\u010Dov\u00E1, Martina" . "CZ - \u010Cesk\u00E1 republika" . . "Alpha-tomatine activates cell cycle checkpoints in the absence of DNA damage in human leukemic MOLT-4 cells"@en . . "11"^^ . "11" . "Alpha-tomatine activates cell cycle checkpoints in the absence of DNA damage in human leukemic MOLT-4 cells" . . "[F065F4AF111B]" . "2" . . "RIV/00216208:11150/13:10130535" . . . "10.2478/v10136-012-0033-8" . . "Alpha-tomatine is a major glycoalkaloid found in the roots, leaves, stems and fruit of tomatoes Lycopersicon esculentum. Recently, alpha-tomatine has been recognized as a potential anticancer drug. In the present study, we identified the signaling cascades involved in the antitumor effect of alpha-tomatine on MOLT-4 leukemic cells. Alpha-tomatine inhibited the proliferation and decreased the viability of MOLT-4 cells in a dose-dependent manner. An increase in the activity of caspases 9 and 3/7 was not observed. However, an increase in the amount of p53 and its phosphorylation on serine 15, as well as an increased amount of mitochondrial protein PUMA was detected 4 and 24 h after exposure to alpha-tomatine at a concentration of 1-3 \u03BCmol/l. Inhibition of the proliferation of MOLT-4 cells by alpha-tomatine is also associated with an increase in p21WAF1/CIP1 and the activation of Chk2. The comet assay did not detect significant amounts of single or double DNA strand breaks in cells treated with alpha-tomatine at concentrations of 0.1-9 \u03BCmol/l. Our results thus contribute to the understanding of the anticancer action of alpha-tomatine." . "1214-021X" . . "http://www.degruyter.com/view/j/jab.2013.11.issue-2/v10136-012-0033-8/v10136-012-0033-8.xml" . "K\u00FAdelov\u00E1, Jana" . . "Alpha-tomatine activates cell cycle checkpoints in the absence of DNA damage in human leukemic MOLT-4 cells" . "RIV/00216208:11150/13:10130535!RIV14-MSM-11150___" . "Alpha-tomatine activates cell cycle checkpoints in the absence of DNA damage in human leukemic MOLT-4 cells"@en . "I" . . "leukemia; PUMA; p53; DNA damage; alpha-tomatine"@en .