. "http://www.sciencedirect.com/science/article/pii/S0003269712000656" . "4"^^ . . . . . "4"^^ . "5"^^ . . "11150" . "123852" . "Spectrofluorometrric metod; Glutathione assay; Glutathione"@en . . "\u010Cervinkov\u00E1, Zuzana" . "RIV/00216208:11150/12:10121250!RIV13-MSM-11150___" . "Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method"@en . . "RIV/00216208:11150/12:10121250" . "[A518B8076DD6]" . "423" . . "I, Z(MSM0021627502)" . . "Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method" . "2" . "Rou\u0161ar, Tom\u00E1\u0161" . "Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method" . . "000302336100008" . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "Ku\u010Dera, Otto" . "10.1016/j.ab.2012.01.030" . "0003-2697" . . "Lotkov\u00E1, Halka" . "Glutathione in an important tripeptide involved in a variety of cellular processes. The aim of our work was to optimize a spectrofluorometric assay for reduced glutathione based on the reaction between glutathione and o-phthalaldehyde. We found these parameters of the method: CV <3%, interasaay CV = 5.1%, recovery = 98-102%, linearity = 0-1000 uM GSH, calibration R2 = 1.00. We also aniticipated possible effect of various amino acids on the fluorescence signal, but no interference was found. We compared the optimized fluorometric method with a popular enzymatic recycling glutathione assay and found very strong correlation of results (r = 0.99, n = 45)" . . . . "Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method"@en . "Glutathione in an important tripeptide involved in a variety of cellular processes. The aim of our work was to optimize a spectrofluorometric assay for reduced glutathione based on the reaction between glutathione and o-phthalaldehyde. We found these parameters of the method: CV <3%, interasaay CV = 5.1%, recovery = 98-102%, linearity = 0-1000 uM GSH, calibration R2 = 1.00. We also aniticipated possible effect of various amino acids on the fluorescence signal, but no interference was found. We compared the optimized fluorometric method with a popular enzymatic recycling glutathione assay and found very strong correlation of results (r = 0.99, n = 45)"@en . "Analytical Biochemistry" . . . .