. . "[C82A305A390F]" . "Central European Journal of Biology" . . "RIV/00216208:11150/10:10072265" . . "\u010Cervinka, Miroslav" . . . "HO-1; Smac/Diablo; ROS; kinases; colon cancer; mitochondria; Selenite"@en . . "285847" . "Z(MSM0021620820)" . "ROS mediate selenite-induced apoptosis in colon cancer cells"@en . . "11150" . "000275141000004" . . "5" . . . . "3"^^ . "Rudolf, Emil" . "Selenite-induced oxidative stress and its relationship to mitochondrial apoptosis was studied in human adenocarcinoma HT-29 cells. It is shown that selenite induces caspase-dependent apoptosis, which is mediated by mitochondria via released cytochrome c, apoptosis-inducing factor (AIF) and Smac/Diablo. Selenite activates stress kinases p38 and JNK while suppressing reduced glutathione (GSH) and thioredoxin reductase (TrxR) levels, transiently inducing heme oxygenase (HO-1) system as well as reducing Akt expression. Pre-treatment of cells with selected antioxidants and stress kinase inhibitors significantly prevented selenite-induced cell death, thereby implicating oxidative stress as a direct (Bax) as well as indirect (via kinases) cause of HT-29 cells demise. These results thus demonstrate for the first time active proapoptotic and anti-survival effects of selenite in colon cancer cells." . . "3"^^ . . "ROS mediate selenite-induced apoptosis in colon cancer cells" . "12"^^ . "RIV/00216208:11150/10:10072265!RIV11-MSM-11150___" . . . "Selenite-induced oxidative stress and its relationship to mitochondrial apoptosis was studied in human adenocarcinoma HT-29 cells. It is shown that selenite induces caspase-dependent apoptosis, which is mediated by mitochondria via released cytochrome c, apoptosis-inducing factor (AIF) and Smac/Diablo. Selenite activates stress kinases p38 and JNK while suppressing reduced glutathione (GSH) and thioredoxin reductase (TrxR) levels, transiently inducing heme oxygenase (HO-1) system as well as reducing Akt expression. Pre-treatment of cells with selected antioxidants and stress kinase inhibitors significantly prevented selenite-induced cell death, thereby implicating oxidative stress as a direct (Bax) as well as indirect (via kinases) cause of HT-29 cells demise. These results thus demonstrate for the first time active proapoptotic and anti-survival effects of selenite in colon cancer cells."@en . "PL - Polsk\u00E1 republika" . . "ROS mediate selenite-induced apoptosis in colon cancer cells"@en . . "Kr\u00E1lov\u00E1, V\u011Bra" . "ROS mediate selenite-induced apoptosis in colon cancer cells" . . . . "2" . "1895-104X" .