. . "8"^^ . . "Moosov\u00E1, Martina" . . "1211-4286" . "3" . "CZ - \u010Cesk\u00E1 republika" . "Pavelkov\u00E1, Jana" . . . . . . . "Mokr\u00FD, Jaroslav" . "8"^^ . . . . "The cultivation of human granulosa cells" . "Br\u016F\u010Dkov\u00E1, Lenka" . "Moos, Ji\u0159\u00ED" . "Acta Medica (Hradec Kr\u00E1lov\u00E9)" . "Experiments with human GCs are mainly focused on the purification of GCs from ovarian follicular fluid followed by FACS analysis or short-term cultivation. The aim of our study was to long-term cultivate GCs, to characterize their morphology and phenotype. Moreover, we have cultivated GCs under gonadotropin stimulation in order to simulate different pathological mechanisms during folliculogenesis (e.g. ovarian hyperstimulation syndrome)." . . "The cultivation of human granulosa cells" . . "The cultivation of human granulosa cells"@en . "[CBC7A5EB1AF4]" . "Soukup, Tom\u00E1\u0161" . "P(NR8932), Z(MSM0021620820), Z(MSM0021627502)" . "Experiments with human GCs are mainly focused on the purification of GCs from ovarian follicular fluid followed by FACS analysis or short-term cultivation. The aim of our study was to long-term cultivate GCs, to characterize their morphology and phenotype. Moreover, we have cultivated GCs under gonadotropin stimulation in order to simulate different pathological mechanisms during folliculogenesis (e.g. ovarian hyperstimulation syndrome)."@en . . . "Kultivace lidsk\u00FDch granulosov\u00FDch bun\u011Bk"@cs . "4"^^ . . "11150" . . "Kultivace lidsk\u00FDch granulosov\u00FDch bun\u011Bk"@cs . . "human granulosa cells; cultivation protocol; proliferation potential"@en . "RIV/00216208:11150/08:00107028!RIV09-MZ0-11150___" . . "V\u00ED\u0161ek, Benjam\u00EDn" . "RIV/00216208:11150/08:00107028" . "361730" . "V\u00FDzkum lidsk\u00FDch granulosov\u00FDch bun\u011Bk je zejm\u00E9na soust\u0159ed\u011Bn na separace t\u011Bchto bun\u011Bk z folikul\u00E1rn\u00ED tekutiny n\u00E1sledov\u00E1n anal\u00FDzou pr\u016Ftokov\u00FDm cytometrem, \u010Di kr\u00E1tkodobou kultivac\u00ED. N\u00E1\u0161 p\u0159\u00EDstup je jin\u00FD. C\u00EDlem na\u0161\u00ED studie byla dlouhodob\u00E1 kultivace lidsk\u00FDch granulosov\u00FDch bun\u011Bk a jejich n\u00E1sledn\u00E1 anal\u00FDza. Nav\u00EDc jsme v pr\u016Fb\u011Bhu kultivace bu\u0148ky stimulovali gonadotropiny s c\u00EDlem navodit r\u016Fzn\u00E9 patologick\u00E9 mechanismy vyskytuj\u00EDc\u00ED se v pr\u016Fb\u011Bhu folikulogeneze (nap\u0159. ovari\u00E1ln\u00ED hyperstimula\u010Dn\u00ED syndrom)."@cs . "The cultivation of human granulosa cells"@en . "\u0158e\u017E\u00E1bek, Karel" . "51" .