. "Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides"@en . . "5" . . "CZ - \u010Cesk\u00E1 republika" . "0009-2770" . "The MALDI-TOF MS is an ideal method for routine identification of proteins separated by two-dimensional polyacrylamide gel electrophoresis (2D-P AGE). However, in some cases the outputs from peptide mass fingerprinting (PMF) do not enable unambiguous identification of proteins. In such situation, determination of a partial amino acid sequence of the target protein would be extremely helpful in the PMF identification. Sequencing using MALDI-TOF post-source decay (PSD) usually generates complex spectra and the fragmentation is not complete. PSD sequencing with chemically assisted fragmentation allows to avoid these problems. Application of both methods in identification of proteins of Francisella tularensis is presented." . "2"^^ . . "Identifikace protein\u016F pomoc\u00ED kombinace proteinov\u00E9 hmotnost\u00ED spektrometrie a fragmentace sulfonovan\u00FDch protein\u016F"@cs . . "RIV/00216208:11150/07:00004060!RIV08-MSM-11150___" . "1"^^ . "425310" . "Stul\u00EDk, Ji\u0159\u00ED" . . . . . . . "MALDI-TOF MS je ide\u00E1ln\u00ED metodou pro rutinn\u00ED identifikaci b\u00EDlkovin separovan\u00FDch dvojrozm\u011Brnou elektrofor\u00E9zou na polyakrylamidov\u00E9m gelu (2D-PAGE). Nicm\u00E9n\u011B v n\u011Bkter\u00FDch p\u0159\u00EDpadech v\u00FDsledky z fingerprintingu peptidov\u00E9 hmoty (PMF) neumo\u017En\u00ED jednozna\u010Dnou identifikaci b\u00EDlkovin. V takov\u00FDch situac\u00EDch by bylo ur\u010Den\u00ED \u010D\u00E1ste\u010Dn\u00E9 aminokyselinov\u00E9 sekvence c\u00EDlov\u00E9 b\u00EDlkoviny u PMF identifikac\u00ED velmi prosp\u011B\u0161n\u00E9. Sekvenace za pomoci MALDI-TOF post-source decay (PSD) obvykle vytv\u00E1\u0159\u00ED komplexn\u00ED spektra a fragmentace nen\u00ED dokon\u010Dena. PSD sekvenace spolu s chemicky podporovanou fragmentac\u00ED umo\u017E\u0148uje se vyhnout t\u011Bmto probl\u00E9m\u016Fm. V n\u00E1sleduj\u00EDc\u00ED \u010D\u00E1sti tohoto sd\u011Blen\u00ED je prezentov\u00E1na aplikace obou metod p\u0159i identifikaci b\u00EDlkovin z Francisely tularensis."@cs . "The MALDI-TOF MS is an ideal method for routine identification of proteins separated by two-dimensional polyacrylamide gel electrophoresis (2D-P AGE). However, in some cases the outputs from peptide mass fingerprinting (PMF) do not enable unambiguous identification of proteins. In such situation, determination of a partial amino acid sequence of the target protein would be extremely helpful in the PMF identification. Sequencing using MALDI-TOF post-source decay (PSD) usually generates complex spectra and the fragmentation is not complete. PSD sequencing with chemically assisted fragmentation allows to avoid these problems. Application of both methods in identification of proteins of Francisella tularensis is presented."@en . "Chemick\u00E9 listy" . "Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides" . . "Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides" . "Identification; Proteins; Combination; Peptide; Fingerprinting; Fragmentation; Sulfonated; Peptides"@en . . "Identifikace protein\u016F pomoc\u00ED kombinace proteinov\u00E9 hmotnost\u00ED spektrometrie a fragmentace sulfonovan\u00FDch protein\u016F"@cs . "S" . . "98" . . "11150" . "4"^^ . "Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides"@en . . . "RIV/00216208:11150/07:00004060" . "[42985FF0EB38]" . . "264;267" . .