"Po ov\u011B\u0159en\u00ED by metodiky mohly podstatn\u011B zrychlit biodosimetrickou diagnostiku a umo\u017Enit efektivn\u011Bj\u0161\u00ED l\u00E9\u010Dbu ANO" . "P(OBUKHK2005001)" . . . "Nov\u00E9 zp\u016Fsoby biodozimetrie a diagnostiky u oz\u00E1\u0159en\u00FDch voj\u00E1k\u016F (BIODOZ) - metodika" . "[EAE4604766C7]" . "V p\u0159\u00EDpad\u011B oz\u00E1\u0159en\u00ED osoby nezn\u00E1mou l\u00E1tkou ionizuj\u00EDc\u00EDho z\u00E1\u0159en\u00ED je nutn\u00E9 odebrat perifern\u00ED krev nejpozd\u011Bji do 6 hodin po oz\u00E1\u0159en\u00ED, kdy je\u0161t\u011B in vivo nejsou posti\u017Een\u00E9 bu\u0148ky fagocytov\u00E1ny. Po vyseparov\u00E1n\u00ED lymfocyt\u016F se provede anal\u00FDza bun\u011Bk: navrhujeme n\u00E1sleduj\u00EDc\u00ED nmo\u017Enosti: 1. Zna\u010Den\u00ED Annexinem a koktejlem monoklon\u00E1ln\u00EDch protil\u00E1tek na detekci subsetu NK bun\u011Bk (anti-CD3, anti-CD8). Porovn\u00E1n\u00EDm poklesu t\u00E9to subpopulace v r\u016Fzn\u00FDch \u010Dasov\u00FDch intervalech by bylo mo\u017En\u00E9 usoudit na velikost obdr\u017Een\u00E9 d\u00E1vky. 2. Zna\u010Den\u00ED H2A.X fosforylovan\u00E9ho na serinu 139 s n\u00E1slednou anal\u00FDzou na pr\u016Ftokov\u00E9m cytometru nebo konfok\u00E1ln\u00EDm mikroskopu. 3. L\u00FDza bun\u011Bk a stanoven\u00ED vzestupu mno\u017Estv\u00ED p21. A\u017E do ov\u011B\u0159en\u00ED na modelu celot\u011Blov\u011B oz\u00E1\u0159en\u00FDch zv\u00ED\u0159at v\u0161ak st\u00E1le plat\u00ED metoda zlat\u00E9ho standardu, kterou je pranace po\u010D\u00EDt\u00E1n\u00ED chromozom\u00E1n\u00EDch aberac\u00ED lymfocyt\u016F oz\u00E1\u0159en\u00E9 osoby."@cs . . "437892" . . "Studie zavedla a adaptovala metodiky pr\u016Ftokov\u00E9 cytometrie (pokles NK bun\u011Bk, kvantifikace gamaH2A.X) a sendvi\u010Dov\u00E9 ELISA (p21, p35_fosfor-ser15) jako potenci\u00E1ln\u00ED biodozimetrick\u00E9 ukazatele analyzuj\u00EDc\u00ED perifern\u00ED lymfocyty." . "Z\u00E1\u0161kodov\u00E1, Darina" . "Vokurkov\u00E1, Doris" . . "Nov\u00E9 zp\u016Fsoby biodozimetrie a diagnostiky u oz\u00E1\u0159en\u00FDch voj\u00E1k\u016F (BIODOZ) - metodika"@cs . "Cerman, Jaroslav" . "V\u00E1vrov\u00E1, Ji\u0159ina" . . . . "RIV/00216208:11150/07:00003881" . "\u0158ez\u00E1\u010Dov\u00E1, Martina" . . "RIV/00216208:11150/07:00003881!RIV08-MO0-11150___" . "Nov\u00E9 zp\u016Fsoby biodozimetrie a diagnostiky u oz\u00E1\u0159en\u00FDch voj\u00E1k\u016F (BIODOZ) - metodika"@cs . . "New methods of biodosimetry and diagnostics in irradiated soldiers - methodology"@en . . . . "Tich\u00FD, Ale\u0161" . "Nov\u00E9 zp\u016Fsoby biodozimetrie a diagnostiky u oz\u00E1\u0159en\u00FDch voj\u00E1k\u016F (BIODOZ) - metodika" . "11150" . . . . . "Univerzita Karlova v Praze, L\u00E9ka\u0159sk\u00E1 fakulta v Hradci Kr\u00E1lov\u00E9, \u00DAstav l\u00E9ka\u0159sk\u00E9 biochemie a Fakultn\u00ED nemocnice Hradec Kr\u00E1lov\u00E9, \u00DAstav klinick\u00E9 imunologie a alergologie" . . "8"^^ . "Metodiky BIODOZ" . . . . . . . "7"^^ . . "Filip, Stanislav" . . "n the case of irradiation by unknown dose of ionizing radiation the sample of peripheral blood must be taken within 6h after the exposure, when the damaged cells are still in circulation. Analysis is performed at separated lymphocytes. We propose following possibilities: 1. Staining by Annexin and mixure of monoclonal antibodies for NK cells subset detection (anti-CD3, anti-CD8). Analysis of decrese of this subset during the time could reflect received dose. 2. Analysis of histone H2A.X phosphorylated on serine 139 by flow-cytometry or confocal microscopy. 3. Lysis of the lyphocytes and detection of increase in protein p21."@en . "V p\u0159\u00EDpad\u011B oz\u00E1\u0159en\u00ED osoby nezn\u00E1mou l\u00E1tkou ionizuj\u00EDc\u00EDho z\u00E1\u0159en\u00ED je nutn\u00E9 odebrat perifern\u00ED krev nejpozd\u011Bji do 6 hodin po oz\u00E1\u0159en\u00ED, kdy je\u0161t\u011B in vivo nejsou posti\u017Een\u00E9 bu\u0148ky fagocytov\u00E1ny. Po vyseparov\u00E1n\u00ED lymfocyt\u016F se provede anal\u00FDza bun\u011Bk: navrhujeme n\u00E1sleduj\u00EDc\u00ED nmo\u017Enosti: 1. Zna\u010Den\u00ED Annexinem a koktejlem monoklon\u00E1ln\u00EDch protil\u00E1tek na detekci subsetu NK bun\u011Bk (anti-CD3, anti-CD8). Porovn\u00E1n\u00EDm poklesu t\u00E9to subpopulace v r\u016Fzn\u00FDch \u010Dasov\u00FDch intervalech by bylo mo\u017En\u00E9 usoudit na velikost obdr\u017Een\u00E9 d\u00E1vky. 2. Zna\u010Den\u00ED H2A.X fosforylovan\u00E9ho na serinu 139 s n\u00E1slednou anal\u00FDzou na pr\u016Ftokov\u00E9m cytometru nebo konfok\u00E1ln\u00EDm mikroskopu. 3. L\u00FDza bun\u011Bk a stanoven\u00ED vzestupu mno\u017Estv\u00ED p21. A\u017E do ov\u011B\u0159en\u00ED na modelu celot\u011Blov\u011B oz\u00E1\u0159en\u00FDch zv\u00ED\u0159at v\u0161ak st\u00E1le plat\u00ED metoda zlat\u00E9ho standardu, kterou je pranace po\u010D\u00EDt\u00E1n\u00ED chromozom\u00E1n\u00EDch aberac\u00ED lymfocyt\u016F oz\u00E1\u0159en\u00E9 osoby." . "New methods of biodosimetry and diagnostics in irradiated soldiers - methodology"@en . . "biodosimetry; ionizing radiation; NK cells; H2A.X; p35; p21; flow cytometry; ELISA"@en . . .